Streptamer® Technology:

Streptamers®: fully reversible reagents for the selection of uncompromised, authentic cells

Cell biology is reliant on the investigation of unmanipulated and uncompromised cells. Unlike state-of-the-art receptor specific cell isolation procedures, the Streptamer® technology offers fully reversible reagents for magnetic cell isolation as well as for fluorescent cell staining. They not only show excellent staining intensities, but they can be removed from the cells by biotin (vitamin H) at low temperatures which results in the following advantages:

  • no stimulation of cells after cell separation
  • optimal preservation of cell effector function
  • improved viability of cells
  • high reproducibility

Uncompromised, authentic cells for research and diagnostics - watch video (in German)

The Streptamer® technology allows both fluorescent staining as well as magnetic isolation of cells with reversible reagents. Virtually all eukaryotic cells can be selected with this technology. The reversible Streptamers® not only show excellent staining intensities, but they can be removed from the cells to optimally preserve cell effector function, making it a versatile platform, not only for basic research, but also for safe and efficient diagnostic use.

Product overview and animation: T cell staining
Fluorescent Fab Streptamers® for fluorescent staining of T cells and subsets, after previous isolation with the magnetic Fab Streptamers®
Fluorescent MHC I Streptamers® for fluorescent staining of antigen-specific CD8+  T cells,  for e.g. FACS

Animation of T cell staining with fluorescent MHC I Streptamers®:

The Streptamer® principle: full reversibility
Low affinity Strep-tagged Fab fragments or MHC molecules are multimerized with Strep-Tactin®, a streptavidin derivative, to generate multimers with high binding avidity.  Strep-Tactin® fluorochromes are used for cell staining / FACS isolation and Strep-Tactin® magnetic beads are used for magnetic cell isolation. Upon addition of low concentrations of d-biotin (Vitamin H), which competes with high affinity for the binding of Strep-tag to Strep-Tactin®, staining and isolation reagents dissociate rapidly from the cell surface.
This reversibility enables multiple cell stainings with different Strep-Tactin® fluorochromes as well as sequential positive cell selections with magnetic beads.
The Streptamer® reagents: a modular system

Fab-Streps or MHC-Streps are either combined with fluorescence- or bead-labeled Strep-Tactin® for cell staining or magnetic isolation, respectively. Biotin is offered as stock solution with the Streptamer® Solution Set which is used for removal of cell staining as well as cell isolation reagents. This modular reagent system enables the economic generation of multiple receptor- or antigen-specific staining and isolation reagents. All reagents can be purchased as single products or assembled in ready-to-use staining and isolation kits.