Receptor-specific Cell Staining - Products

Fluorescent Fab Streptamers®, adducts of Strep-tagged Fab fragments (Fab-Streps) and Strep-Tactin® PE or APC, are directed against cell surface markers including those of T cells (CD3, CD4, CD8), memory T cells (CD8, CD62L, CD45RA-), regulatory T cells (CD4, CD25, CD45RA).

Cell staining procedure

1) Fab-Streps (Strep-tagged Fab fragments) are multimerized with Strep-Tactin® attached to a fluorochrome to generate multimers with high binding avidity. These multimers are directed to the respective cell surface marker(s) and are thus determined for staining of the target cell (Fig.1).

Fab Streptamer fluorochrome
Fig.1: Fab Streptamer® Fluorochrome

2) After FACS analysis or FACS sorting of the target cells, the staining reagents can be completely removed with biotin (Fig.2). Cells are left phenotypically and functionally indistinguishable from untreated cells.

Fig. 2: Target cells are selected by a Fab Streptamer®. After staining and fluorescence assisted sorting, the Strep-Tactin® complex is removed from the target cells by the addition of biotin under physiological conditions at low temperatures (4°C). The monomeric low affinity Fab-Strep molecules are then dissociated completely from the cell surface by two short washing steps. The isolated cells are phenotypically and functionally undistinguishable from non-treated cells and predestined for clinical use.

Please note: for receptor-specific cell staining, your will need the Fab-Strep in our Fab Streptamer magnetic cell selection kits, please visit the product page

Product Contents Cat.no Price   Order
Strep-Tactin® PE for Fab Streptamers for 5x10^7 to 1 x 10^8 cells 6-5001-005 USD 366.00
6-5001-010ws

For fluorescence-staining of cell isolates obtained after magnetic Fab Streptamer cell isolation. All required reagents for cell isolation, except Strep-Tactin PE or APC, are included in the Fab Streptamer isolation kits. ...

Further details, manuals, data sheets, related products and more
Strep-Tactin® APC for Fab Streptamers for 5x10^7 to 1x10^8 cells 6-5011-005 USD 366.00
6-5011-010ws

For fluorescence-staining of cell isolates obtained after magnetic Fab Streptamer cell isolation. All required reagents for cell isolation, except Strep-Tactin PE or APC, are included in the Fab Streptamer isolation kits. ...

Further details, manuals, data sheets, related products and more