Fluorescent staining / FACS of Lymphocytes (T cells)

Strep-Tactin^® PE or APC for fluorescent staining / FACS sorting of T cell isolates which are obtained after magnetic cell isolation with the Fab Streptamer^® isolation kits.

What you need:

• For previous magnetic cell isolation: Fab Streptamer^® Isolation Kit MB of choice, StrepMan magnet
• For fluorescent staining of cell isolates: Strep-Tactin^® PE or APC

General procedure:

1. Isolate target cell with the respective Fab Streptamer^® Isolation Kit
2. Stain cell isolate with Strep-Tactin^® PE or APC (see staining workflow below)