References about detection

Strep-tag review

Schmidt, T.G.M. and Skerra, A. (2007)
The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins.
Nature Protocols 2, 1528-1535.

Tag comparison
  1. Vasilescu J. and Figeys D. (2006)
    Mapping protein–protein interactions by mass spectrometry. 
    Current Opinion in Biotechnology 17, 394–399.
  2. Lichty, J.J., Malecki, J.L., Agnew, H.D., Michelson-Horowitz, D.J. and Tan, S. (2005) 
    Comparison of affinity tags for protein purification. 
    Prot. Expr. Purific. 41, 98-105.
  3. Cass, B., Pham, P.L., Kamen, A. and Durocher, Y. (2004) (double-tag, Strep-His-tag)
    Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme. 
    Prot. Expr. Purific. 40, 77-85.
  4. Witte, C.P., Noël, L.D., Gielbert, J., Parker, J.E. and Romeis, T. (2004) (Plant expression)
    Rapid one-step protein purification from plant material using the eight-amino acid strepII epitope. 
    Plant Molec. Biol. 55, 135-147.
  5. Terpe, K. (2003) 
    Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. 
    Appl. Microbiol. Biotechnol. 60, 523-53.


  1. Müller I. B., Knöckel J., Groves M. R., Jordanova R., Ealick S. E., Walter R.D., Wrenger C. (2008)
    The Assembly of the Plasmodial PLP Synthase Complex Follows a Defined Course. 
    PlosOne 3 (3), e1815.
  2. Ichioka F., Kobayashi R., Katoh K., Shibata H. and Maki M. (2008)
    Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4). 
    FEBS Journal 275 (4), 682-692.
  3. Kurdyukov S., Faust A., Nawrath C., Bär S., Voisin D., Efremova N., Franke R., Schreiber L., Saedler H., Métraux J.-P., and Yephremova A. (2006)
    The Epidermis-Specific Extracellular BODYGUARD Controls Cuticle Development and Morphogenesis in Arabidopsis. 
    The Plant Cell 18, 321–339.
  4. Rummel, A., Mahrhold, S., Bigalke H. and Binz, T. (2003) 
    The HCC-domain of botulinum neurotoxins A and B exhibits a singular ganglioside binding site displaying serotype specific carbohydrate interaction. 
    Molec. Microbiology 51, 631-643
  5. Ribrioux, S., Kleymann, G., Haase, W., Heitmann, K., Ostermeier, C. and Michel, H. (1996) 
    Use of nanogold- and fluorescent-labeled antibody Fv fragments in immunochemistry. 
    The Journal of Histochemistry and Cytochemistry 44, 207-213.
  6. Kleymann, G., Iwata, S., Wiesmüller, H.H., Ludwig, B. and Michel, H. (1995) 
    Immunoelectronic microscopy and epitope mapping with monoclonal antibodies suggests the existence of an additional N-terminal transmembrane helix in the cytochrome b subunit of bacterial ubiquinol:cytochrome-c oxidoreductases. 
    Eur. J. Biochem. 230, 359-363.
  7. Kleymann, G., Ostermeier, C., Heitmann, K., Haase, W. and Michel H. (1995) 
    Use of antibody fragments (Fv) in immunochemistry. 
    The Journal of Histochemistry and Cytochemistry 43, 607-614.
  8. Tsiotis, G., Haase, W., Engel, A. and Michel, H. (1995) 
    Isolation and structural characterization of trimeric cyanobacterial photosystem I complex with the help of recombinant antibody fragments. 
    Eur. J. Biochem. 231, 823-830.
  1. Ernst, W.J., Spenger, A., Toellner, L., Katinger, H. and Grabherr, R.M. (2000) 
    Expanding baculovirus surface display modification of the native coat protein Gp64 of autographa californica NPV. 
    Eur. J. Biochem. 267, 4033-4039.
  2. Panke, O., Gumbiowski, K., Junge, W. and Engelbrecht, S. (2000) 
    F-ATPase: specific observation of the rotating c subunit oligomer of EFoEF1. 
    FEBS Letters 472, 34-38.
  3. Skerra, A. and Schmidt, T.G.M. (1993) 
    Use of the strep-tag and streptavidin for detection and purification of recombinant proteins. 
    Methods in Enzymology 326, 271-304.
  4. Sebastian, P., Wallwitz, J. and Schmidt, S. (2003) 
    Semi automated production of a set of different recombinant GST-streptag fusion proteins.
    J. Chromatogr. B 786, 343-355