Applications

Capture of functional target proteins on Strep-Tactin® coated microplates

Determination of an alkaline phosphatase (AP) Strep-tag® II fusion protein

Capture of Strep-tag® II fusion protein onto Strep-Tactin® coated microplates
1. Strep-Tactin® coated microplate was incubated with different amounts of recombinant E. coli alkaline phosphatase/Strep-tag® II fusion protein. 2. Washing cycles. 3. Colorimetric determination of bound AP-activity.
H. pylori urease as antigen in a solid phase immunoassay
General conditions:
  1. Strep-Tactin® coated microplate was incubated with recombinant H. pylori urease/Strep-tag® II fusion protein, followed by 3 washing cycles.
  2. Second incubation with human sera followed by 3 washing cycles.
  3. Third incubation with rabbit anti-human lgG conjugated to peroxidase, followed by 3 washing cycles.
  4. Colorimetric determination of bound peroxidase activity.

Comparison of Strep-Tactin®XT and Strep-Tactin® coated microplates