Capture of functional target proteins on Strep-Tactin® coated microplates
H. pylori urease as antigen in a solid phase immunoassay
- Strep-Tactin® coated microplate was incubated with recombinant H. pylori urease/Strep-tag® II fusion protein, followed by 3 washing cycles.
- Second incubation with human sera followed by 3 washing cycles.
- Third incubation with rabbit anti-human lgG conjugated to peroxidase, followed by 3 washing cycles.
- Colorimetric determination of bound peroxidase activity.