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GFP Selector for immunoprecipitation of GFP-tagged proteins

GFP Selector Resin

GFP Selector features high affinity and superior capacity for immunoprecipitation experiments of GFP fusion proteins while showing negligible non-specific background. GFP Selector immobilizes a wide range of GFP derivatives. For details see our Product Specificity Chart below.

GFP Selector Resin Application

GFP Selector is compatible with physiological buffers and with high stringency buffers (see Product Data Sheet). GFP Selector provides great freedom to adjust the binding and washing conditions to the experimental needs.

GFP Selector Products

Product	Contents	Cat.no	Price	Order
GFP Selector	200µl Slurry for 10 reactions	2-9131-002	USD 163.00	Quantity:
GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GFP Selector ... Further details, manuals, data sheets, related products and more
GFP Selector	500µl Slurry for 25 reactions	2-9131-005	USD 338.00	Quantity:
GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GFP Selector ... Further details, manuals, data sheets, related products and more
GFP Selector	2000µl slurry for 100 reactions	2-9131-020	USD 1,235.00	Quantity:
GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GFP Selector ... Further details, manuals, data sheets, related products and more
GFP Selector Kit	1 Kit	2-9132-008	USD 423.00
Our GFP Selector Kit contains 500 µl GFP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 2x10 MiniSpin columns. Further details, manuals, data sheets, related products and more

GFP Selector Specifications

Specificity: Recognizes GFP, mEGFP, superfolder GFP, Emerald GFP and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. For more detailed information see data sheet (click on product).
Capacity: > 4 µg GFP per µl of packed beads (i.e. 2 µl of slurry)
Support: 4% cross-linked agarose beads
Formulation: 50% slurry in 20% ethanol / PBS
Recommended for: Immunoprecipitation, Mass Spectrometry
GFP Selector Kit: Our GFP Selector Kit contains 500 µl GFP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 2x10 MiniSpin columns.

Source: Nanotag Biotechnologies GmbH

A, Pull-down of GFP from a mixture of GFP, mCherry and mTagBFP

B, Immunoprecipitation of GFP (•) from HeLa lysate.
    In/Ft: 1/1000 of input and non-bound material.
    E: Eluate from 1 µl of beads
    * Specific maturation band from GFP family members

C, Control experiment using functionalized beads lacking sdAbs.