GFP Selector for immunoprecipitation of GFP-tagged proteins
GFP Selector features high affinity and superior capacity for immunoprecipitation experiments of GFP fusion proteins while showing negligible non-specific background. GFP Selector immobilizes a wide range of GFP derivatives. For details see our Product Specificity Chart below.
GFP Selector is compatible with physiological buffers and with high stringency buffers (see Product Data Sheet). GFP Selector provides great freedom to adjust the binding and washing conditions to the experimental needs.
GFP Selector Products
|GFP Selector||2 ml||2-9131-020||USD 335.00|
GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GFP Selector ...
Recognizes GFP, mEGFP, superfolder GFP, Emerald GFP and most common
CFP and YFP variants. It does not cross-react with mCherry, mRFP,
dsRed, mTagBFP or their most common derivatives. For more detailed
information see data sheet (click on product).
Capacity: > 4 µg GFP per µl of packed beads (i.e. 2 µl of slurry)
Support: 4% cross-linked agarose beads
Formulation: 50% slurry in 20% ethanol / PBS
Recommended for: Immunoprecipitation, Mass Spectrometry
In/Ft: 1/1000 of input and non-bound material.
E: Eluate from 1 µl of beads
* Specific maturation band from GFP family members