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MBP Selector for immunoprecipitation of MBP-tagged proteins

MBP Selector Resin
MBP Selector Resin

 

MBP Selector features high affinity and superior capacity for immunoprecipitation experiments of MBP fusion proteins while showing negligible non-specific background.

MBP Selector is compatible with physiological buffers and high stringency buffers. MBP Selector provides great freedom to adjust the binding and washing conditions to the experimental needs.

 

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MBP Selector Products

Product Contents Cat.no Price   Order
MBP Selector 200µl for 10 reactions 2-9111-002 USD 163.00  
MBP-Selector

MBP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. MBP Selector thus features high affinity and superior capacity for MBP fusion proteins while showing negligible non-specific background.

Further details, manuals, data sheets, related products and more
MBP Selector 500µl for 25 reactions 2-9111-005 USD 338.00  
MBP-Selector

MBP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. MBP Selector thus features high affinity and superior capacity for MBP fusion proteins while showing negligible non-specific background.

Further details, manuals, data sheets, related products and more
MBP Selector 2000µl slurry for 100 reactions 2-9111-020 USD 1,235.00  
MBP-Selector

MBP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. MBP Selector thus features high affinity and superior capacity for MBP fusion proteins while showing negligible non-specific background.

Further details, manuals, data sheets, related products and more
MBP Selector Kit 1 Kit 2-9112-008 USD 423.00  
SelectorResinKit

Our MBP Selector Kit contains 500 µl MBP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 2x10 MiniSpin columns.

Further details, manuals, data sheets, related products and more
MBP Selector Specifications

Specificity: Recognizes E.coli maltose-binding protein (MBP)
Support: 4% cross-linked agarose beads
Capacity: > 2.5 µg MBP per µl of packed beads (i.e. 2 µl of slurry)
Formulation: 50% slurry in 20% ethanol / PBS
Recommended for: Immunoprecipitation, Mass Spectrometry
MBP Selector Kit: Our MBP Selector Kit contains 500 µl MBP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 2x10 MiniSpin columns.

Immunoprecipitation of MBP from HeLa lysate
Source: Nanotag Biotechnologies GmbH

 

Immunoprecipitation of MBP (•) from HeLa lysate.

In/Ft: 1/1000 of input and non-bound material.
E: Eluate from 1 µl of beads
Right panel: Control experiment using functionalized beads lacking sdAbs.