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RFP Selector for immunoprecipitation of RFP-tagged proteins

RFP Selector Resin
RFP Selector Resin

 

RFP Selector features high affinity and superior capacity for immunoprecipitation experiments of RFP fusion proteins while showing negligible non-specific background. RFP Selector immobilizes a wide range of RFP derivatives including mCherry. For details see our product specifications below.

RFP Selector is compatible with physiological buffers and with high stringency buffers. RFP Selector provides great freedom to adjust the binding and washing conditions to the experimental needs.

 

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RFP Selector Products

Product Contents Cat.no Price   Order
RFP Selector 200µl Slurry for 10 reactions 2-9141-002 USD 163.00  
RFP-Selector

RFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. RFP Selector ...

Further details, manuals, data sheets, related products and more
RFP Selector 500µl Slurry for 25 reactions 2-9141-005 USD 338.00  
RFP-Selector

RFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. RFP Selector ...

Further details, manuals, data sheets, related products and more
RFP Selector 2000µl slurry for 100 reactions 2-9141-020 USD 1,235.00  
RFP-Selector

RFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. RFP Selector ...

Further details, manuals, data sheets, related products and more
RFP Selector Kit 1 Kit 2-9142-008 USD 423.00  
SelectorResinKit

Our RFP Selector Kit contains 500 µl RFP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 2x10 MiniSpin columns.

Further details, manuals, data sheets, related products and more
RFP Selector Specifications

Specificity: Recognizes most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato and mScarlet. Does not cross-react with GFP or mTagBFP/mTagRFP derivatives. For more detailed information see data sheet (click on product).
Support: 4% cross-linked agarose beads
Capacity: > 4 µg RFP per µl of packed beads (= 2µl of slurry)
Formulation: 50% slurry in 20% ethanol / PBS
Recommended for: Immunoprecipitation, Mass Spectrometry
RFP Selector Kit: Our RFP Selector Kit contains 500 µl RFP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 2x10 MiniSpin columns.

Pull-down of mCherry from a mixture of GFP, mCherry and mTagBFP
Source: Nanotag Biotechnologies GmbH
 
A, Pull-down of mCherry from a mixture of GFP, mCherry and mTagBFP
B, Immunoprecipitation of mCherry (•) from HeLa lysate
    In/Ft: 1/1000 of input and non-bound material
    E: Eluate from 1 µl of beads
    * Specific maturation band from RFP family members
    (see e.g. Gross et al. PNAS (2000) 11990-11995)
C, Control experiment using functionalized beads lacking sdAbs