TagFP Selector for immunoprecipitation of mTagBFP-tagged proteins
TagFP Selector features high affinity and superior capacity for immunoprecipitation experiments of TagFP fusion proteins while showing negligible non-specific background.
TagFP Selector is compatible with physiological buffers and high stringency buffers. TagFP Selector provides great freedom to adjust the binding and washing conditions to the experimental needs.
TagFP Selector Products
|TagFP Selector||2 ml||2-9151-020||USD 335.00|
TagFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. TagFP Selector ...
Recognizes mTagBFP, mTagRFP, mTagRFP657 and mKate. Reactivity
against other TagRFP derivatives not analyzed. Does not cross-react
with common GFP- or dsRed/RFP derivatives including EBFP. For more
detailed information see data sheet (click on product).
Support: 4% cross-linked agarose beads
Capacity: > 4 µg BFP per µl of packed beads (= 2µl of slurry)
Formulation: 50% slurry in 20% ethanol / PBS
Recommended for: Immunoprecipitation, Mass Spectrometry
In/Ft: 1/1000 of input and non-bound material.
E: Eluate from 1 µl of beads
* Specific maturation band from BFP family members