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TagFP Selector for immunoprecipitation of mTagBFP-tagged proteins

TagFP Selector Resin
TagFP Selector Resin

 

TagFP Selector features high affinity and superior capacity for immunoprecipitation experiments of TagFP fusion proteins while showing negligible non-specific background.

TagFP Selector is compatible with physiological buffers and high stringency buffers. TagFP Selector provides great freedom to adjust the binding and washing conditions to the experimental needs.

 

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TagFP Selector Products

Product Contents Cat.no Price   Order
TagFP Selector 200µl Slurry for 10 reactions 2-9151-002 USD 163.00  
TagFP-Selector

TagFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. TagFP Selector ...

Further details, manuals, data sheets, related products and more
TagFP Selector 500µl Slurry for 25 reactions 2-9151-005 USD 338.00  
TagFP-Selector

TagFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. TagFP Selector ...

Further details, manuals, data sheets, related products and more
TagFP Selector 2000µl slurry for 100 reactions 2-9151-020 USD 1,235.00  
TagFP-Selector

TagFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. TagFP Selector ...

Further details, manuals, data sheets, related products and more
TagFP Selector Kit 1 Kit 2-9152-008 USD 423.00  
SelectorResinKit

Our TagFP Selector Kit contains 500 µl TagFP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 2x10 MiniSpin columns.

Further details, manuals, data sheets, related products and more
TagFP Selector Specifications

Specificity: Recognizes mTagBFP, mTagRFP, mTagRFP657 and mKate. Reactivity against other TagRFP derivatives not analyzed. Does not cross-react with common GFP- or dsRed/RFP derivatives including EBFP. For more detailed information see data sheet (click on product).
Support: 4% cross-linked agarose beads
Capacity: > 4 µg BFP per µl of packed beads (= 2µl of slurry)
Formulation: 50% slurry in 20% ethanol / PBS
Recommended for: Immunoprecipitation, Mass Spectrometry
TagFP Selector Kit: Our TagFP Selector Kit contains 500 µl TagFP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 2x10 MiniSpin columns.

Pull-down of mTagBFP from a mixture of GFP, mCherry and mTagBFP
Source: Nanotag Biotechnologies GmbH
 
A, Pull-down of mTagBFP from a mixture of GFP, mCherry and mTagBFP
B, Immunoprecipitation of mTagBFP (•) from HeLa lysate.
    In/Ft: 1/1000 of input and non-bound material.
    E: Eluate from 1 µl of beads
    * Specific maturation band from BFP family members
C, Control experiment using functionalized beads lacking sdAbs.