Home > mCD3 and mCD4 T cell separation

Cell Separation with Fab-TACS® technology - without magnetic labeling!

The fragment antigen-binding (Fab) traceless affinity cell selection (TACS) technology allows a convenient and easy way for cell separation. The desired cells can be selected directly from mouse blood, spleen, brain and lymph nodes. These cells are entirely free of microbead and antibody contamination. The positive separation of the target cells by Fab-TACS® technology is fully reversible. The purified cells are ready for in vivo use and further downstream applications. Read more about the technology here.

 

Fab-TACS® Gravity columns for mouse CD3 and mouse CD4 cells

Test it yourself!

Send an email with your contact details to

fab-tacs@iba-lifesciences.com

and order your free mCD3/mCD4 Isolation Introductory Kit*

(find detailed information on the introductory kit here).

*Includes : 2x Fab-TACS® Gravity columns (Capacity: up to 1x108 mouse target cells each), 2x Fab-Strep of  your choice (2x mCD3, 2x mCD4 or mCD3 + mCD4 Fab-Strep), Buffer CI (10x), Biotin Stock Solution. Distributors and IBA staff excluded. Free samples are limited to one per person/lab, only while supplies last. Free shipping to EU countries only. Valid until June 30th 2019.

Cell separation workflow with Fab-TACS®

cell separation workflow with fab-tacs®

Separation of CD4 T cells from mouse splenocytes

Positive separation of mCD4 T cells

Figure: Positive isolation of mouse CD4+ T cells from splenocytes.

Fab-TACS® technology results in higher yield, purity and viability in comparison to a competitor product. The bar chart indicates mean values of isolated target cells from three independent samples.

Click the chart to enlarge.

See how the Fab-TACS® Gravity technology works

Cell isolation with Fab-TACS® Gravity

Separating cells without magnetic or antibody labeling. The traceless affinity cell selection (Fab-TACS®) is a gentle selection process, which delivers label-free non-activated target cells of highly reproducible quality and purity directly from a variety of single cell suspensions. This process allows a multitude of downstream applications. 

Target cells are captured by cognate Fab fragments that are reversibly immobilized on a column matrix via an epitope tag. Low affinity Fab fragments are attached to a cell grade agarose matrix coated with Strep-Tactin®. Multimerization promotes the binding to target cells with high avidity. The target cells adhere to the affinity matrix. Non-target cells are washed away efficiently. Biotin triggers the elution of the target cells due to the reduced affinity of the Fab-Streps. 

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