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Manual magnetic isolation of antigen-specific (CD8+) T cells

Reversible MHC I Streptamers® for positive selection of antigen-specific CD8+ T cells from PBMCs with magnetic microbeads and magnet.

What you need:

Labelling of target cells (cells of interest) with MHC I Streptamers®

Workflow of magnetic cell isolation with MHC I Streptamers® - Labelling

Cell isolation via MHC I Streptamers®

Magnetic cell isolation with MHC I Streptamers®

Removal of MHC I Streptamers® from target cells

Removal of MHC I Streptamers® from isolated cells
MHC I Streptamer® based cell isolation results in functional and label free cells of intererest

Magnetic MHC I Streptamer®

Components of the magnetic MHC I Streptamer® complex
Components of the magnetic MHC I Streptamer® complex
Summary MHC I Streptamers®

Magnetic MHC I Streptamer®

Reagents:
  • Antigen-specific MHC I-Streps; choose among a large range of different alleles and antigens
  • Strep-Tactin® magnetic microbeads
  • Buffer IS, 10x solution
  • Biotin stock solution