References for cell isolation with Fab Streptamers®

CAR T cell enrichment

Sabatino, M.; Hu, J.; Sommariva, M.; Gautam, S.; Fellowes, V,; Hocker, J.D.; Dougherty, S.; Qin, H.; Klebanoff, C.A.; Fry, T.J.; Gress, R.E.; Kochenderfer, J.N., Stroncek, D.F. Ji, Y. and Gattinoni, L.  (2016) Generation of clinical-grade CD19-specific CAR-modified CD8+ memory stem cells for the treatment of human B-cell malignancies Blood DOI 10.1182/blood-20105-11-683847 [Link]

Serial positive enrichment / multiparameter cell sorting

Stemberger, C.; Dreher, S.; Tschulik, C.; Piossek, C.; Bet, J.; Yamamoto, T. N.; Schiemann, M.; Neuenhahn, M.; Martin, K.; Schlapschy, M.; Skerra, A.; Schmidt, T.; Edinger, M.; Riddell, S.; Germeroth, L. and Busch, D.  (2012) Novel serial positive enrichment technology enables clinical multiparamenter cell sorting. PLoS One7 (4), 1-11 [Link]

Full reversibility and preserved function of isolated cells

Reversible Streptamers® confer full protection against L. monocytogenes infection in mice (Knabel et al., 2002)

The reversible Streptamers® maintain the in vivo function of isolated cells. In contrast, conventional MHC multimers/tetramers signifcantly change the phenotype and function of stained T cells at physiological temperatures (Knabel et al., 2002).

Pretreatment of LLO91-99-specific T cells with conventional binding MHC tetramer reagents resulted in significantly reduced protection towards Listeria infection following adoptive transfer. In contrast, after complete removal of the Streptamers® with biotin, the same number of cells conferred almost an identical degree of protection as compared with positive controls.

Without removal of the Streptamers® before adoptive cell transfer, the cells were reproducibly more effective than cells coated with conventional MHC tetramers. Please read the complete paper Knabel et al., 2002.

Reversible Streptamers® preserve proliferation capacity and functional status of CD8+ T cells (Wang et al., 2013)

Proliferation of CMVpp65-specific CD8+ T lymphocytes was preserved after selection with reversible Streptamers®, while it might have been altered with tetramers (Fig.3). Furthermore, the functional status as measured by activity (secretion of IFN-gamma) and cytotoxic effectiveness (secretion of granzyme B) remained very active when selected with Streptamers® as compared to tetramers (Fig.4).

Reversible Streptamers® preserve T cell receptor expression of CD8+ T cells (Zhang et al., 2016)