Manual magnetic isolation of Lymphocytes (T cells)
Reversible Fab Streptamers® for (serial) positive selection(s) of T cells and subsets from PBMCs with magnetic microbeads and a hand-held magnet.
What you need:
Magnetic Fab Streptamer®
Components of the magnetic Fab Streptamer® complex
StrepMan magnet
Selection of the target cells is performed with our StrepMan permanent magnet. We recommend a horizontal position for separation of the magnetically labeled cells (a). For removal of the magnetic Streptamers® from the isolated cells with biotin we recommend an upright position of the StrepMan magnet (b).
Components:
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Marker-specific Fab-Streps (up to three different for serial positive selection)
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Strep-Tactin® magnetic microbeads
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Buffer IS, 10x solution
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Biotin stock solution
