Magnetic T cell isolation with Fab Streptamers®
1) Fab-Streps (= Strep-tagged Fab fragments) which recognize the surface markers
2) Magnetic beads (= Strep-Tactin® magnetic beads) which reversibly bind the Fab-Streps
For cell isolation with Fab-Streps we offer microbeads (Strep-Tactin® magnetic microbeads) and our hand-held StrepMan permanent magnet (see below).
1) Fab-Streps (Strep-tagged Fab fragments) are multimerized with Strep-Tactin® attached to a magnetic bead to generate multimers with high binding avidity (Fig.1).
2) Selection of the target cells is performed with our StrepMan permanent magnet. We recommend a horizontal position for separation of the magnetically labeled cells (top). For removal of the magnetic Streptamers from the isolated cells with biotin we recommend an upright position of the StrepMan magnet (bottom). Cells are left phenotypically and functionally indistinguishable from untreated cells.