5'-Non-nucleosidic modifications

5'-Non-nucleosidic modifications 5' 3' Internal Cat. no. Comments
5'-C9-Alkyne   5-0209-01X For alkyne modification of oligos
5'-C9-Alkyne-CEP

5'-C9-Alkyne is used for alkyne modification of oligos that can then be labeled or modified by click chemistry.

5'-C15-Alkyne   5-0209-02X For alkyne modification of oligos
5'-C15-Alkyne-CEP

5'-C15-Alkyne is used for alkyne modification of oligos that can then be labeled or modified by click chemistry.

5'-Alkyne-PEG3   5-0209-00X For alkyne modification of oligos
5'-Alkyne-PEG3-CEP

5'-Alkyne-PEG3 is used for alkyne modification of oligos that can then be labeled or modified by click chemistry.

5'-DBCO-PEG4   5-0209-10X For copper-free Click reactions
DBCO-PEG4
5'-Amino-C3   5-0210-10X For attachment of dyes and modifications
5aminoc3
  • Primary amino group attached to a 3-carbon spacer
  • Useful for the attachment of fluorescent reporter groups, biotin, digoxigenin, and enzymes or antibodies
  • Used for immobilization of oligonucleotides on solid supports therefore used commonly in DNA arrays and solid-phase PCRs
  • Used when the oligonucleotide must not be spaced far from the corresponding interaction.
5'-Amino-C6   5-0210-11X For attachment of dyes and modifications
5AminoC6
  • Primary amino group attached to a 6-carbon spacer
  • Useful for the attachment of fluorescent reporter groups, biotin,    digoxigenin, and enzymes or antibodies 
  • Used for immobilization of oligonucleotides on solid supports
5'-Amino-C12   5-0210-21X For attachment of dyes and modifications
5AminoC12
  • Primary amino group attached to a 12-carbon spacer
  • Greater spacing between oligonucleotide and reporter groups
  • Can reduce intramolecular quenching of fluorescence
  • Useful for affinity chromatography
5'-Branching (5’-Branch)   5-0210-22X Multiplies attachment sites
5Branching
  • Each coupling doubles the amount of sites used for coupling with modifications
5’-Carboxyl   5-0210-23X For immobilization and coupling
  • Used for immobilization of oligonucleotides on solid supports
  • Used for coupling of oligonucleotides with proteins
5'-Cholesteryl (5’-Chol)   5-0210-31X For antisense technology
5Cholesteryl
  • Useful in antisense technology
  • The highly lipophilic compound facilitates membrane penetration
5'-Hexaethylenglycol (5'-HeGl)   5-0210-41X 18-Atom spacer
5Hexaethylenglycol
  • Hydrophilic spacer
  • Can also be introduced at internal sites
  • In hammerhead ribozymes it can replace the tetra-loop of stem II with essentially no decrease in activity compared with the wild-type ribozyme
5'-Phosphate (5'-Phos)   5-0210-51X For ligation
  • For ligation of PCR products
  • Very high degree of phosphorylation (>95%)
  • Superior to enzymatic methods
5'-Psoralen-C2 (5'-PsorC2)   5-0210-61X For nucleic acid cross-linking
5Psoralen
  • For cross-linking double-stranded oligonucleotides
5'-Psoralen-C6 (5'-PsorC6)   5-0210-71X For cross-linking of triple helices
5Psoralen
  • For cross-linking triple helices
5'-Thiol-C6 (5'-SH)   5-0210-81X For attachment of dyes and immobilisation
5ThiolC6
  • For coupling oligonucleotides with maleimide or iodoacetate activated antibodies, peptides, enzymes or fluorescent dyes
  • Useful for double labelings with different dyes (5'-thiol in combination with internal- or 3'-amino-groups)  
5'-Triethylenglycol (5'-TriGl)   5-0210-91X 9-Atom spacer
5Triethylenglycol
  • Hydrophilic spacer
  • Can also be coupled at internal sites
*: All Oligos are available in 0,05; 0,2; 1,0 µmol
**: All modified DNA oligonucleotides are HPLC purified at no additional charge.
To order your tailor-made oligo above get in contact with our individual personal assistance provided by nucleic acid chemists and molecular biologists. Your personal contact person from IBA Lifesciences is ready to help you by email or phone (+49 551 50672-222) and will support you in designing your correct oligo sequence and advise you in possible promising labeling and modification combinations. Furthermore our assistance alerts you to create incorrect not functional oligonucleotides before starting the synthesis.