Non-nucleosidic modifications

Non-nucleosidic modifications 5' 3' Internal Cat. no. Comments
Biotin 5-0320-0XX Hapten marker
5Biotin

Biotin is a very popular hapten marker for oligonucleotides. An extensive variety of products are available for the detection and immobilization of biotin labeled probes. As biotin has a high affinity to streptavidin, the detection of biotin labeled probes is very sensitive. Examples of this are in situ hybridization, cDNA synthesis with biotin oligo-dT, capture of amplified DNA sequences and solid phase sequencing. Biotin can be introduced either at the 3'-end and/or at the 5'-end and at internal sites at any thymidine residue. For best results and highest sensitivity the distance between each biotin in the probe should be at least 15 bases. Every biotin labeled oligonucleotide is carefully analyzed by HPLC for quantitative labeling. Now also available for Click-Chemistry!

  • Structure on the right: 5'- Biotin
Iminobiotin   5-0322-0XX Analogue of biotin
5Iminobiotin

Mild purification conditions for active reporter molecules in e.g. DNA/DNA or DNA/protein binding studies
2-Iminobiotin, an analogue of biotin, interacts with avidin and streptavidin in a pH-dependent manner allowing recovery of your reporter molecule coupled to iminobiotin. At high pH, the free base form of 2-iminobiotin retains the high-affinity specific binding characteristics of biotin whereas at acidic pH values the salt form of the analogue interacts poorly with avidin. Using these properties for an affinity purification protocol you will be able to load your biomolecule to an avidin matrix at pH 9.5 and elute it at pH 4, thus, overcoming the harsh elution conditions associated with biotin.

We offer

  • oligonucleotides labeled with iminobiotin or biotin 
  • carefully analyzed by HPLC for quantitative labeling   
  • labels introduced at the 3´-end, 5´-end and internally at any thymidine residue 
  • Structure on the right: 5'- Iminobiotin
Desthiobiotin 5-0322-0XX Derivative of biotin that lacks a sulfur atom
5Desthiobiotin

Desthiobiotin is a biotin derivative that binds to streptavidin with a lower affinity than biotin.Desthiobiotin-labeled oligonucleotides can be eluted from streptavidin by biotin via affinity purification methods.

  • Structure on the right: 5'- Desthiobiotin
Digoxigenin 5-0321-0XX Hapten with low endogenous background
5Digoxigenin

Digoxigenin (Dig*) is a hapten occuring naturally only in Digitalis purpurea. This explains the low endogenous background associated in applications with Dig. A range of conjugated anti-Dig antibodies are available, such as alkaline phosphatase, peroxidase, fluorescein and rhodamine. Dig can be introduced either at the 3'-end and/or the 5'-end as well as at internal sites at any thymidine residue. For best results and highest sensitivity the distance between each Dig in the probe should be at least 15 bases.Every Dig labeled oligonucleotide is carefully analyzed by HPLC for quantitative labeling.

  • Structure on the right: 5'- Digoxigenin
To order your tailor-made oligo above get in contact with our individual personal assistance provided by nucleic acid chemists and molecular biologists. Your personal contact person from IBA Lifesciences is ready to help you by email or phone (+49 551 50672-222) and will support you in designing your correct oligo sequence and advise you in possible promising labeling and modification combinations. Furthermore our assistance alerts you to create incorrect not functional oligonucleotides before starting the synthesis.