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Technical support & information

Get in contact with our individual personal assistance provided by nucleic acid chemists and molecular biologists. Your personal contact person from IBA Lifesciences is ready to help you by email or phone (+49 551 50672-222) and will support you in designing your correct oligo sequence and advise you in possible promising labeling and modification combinations. Furthermore our assistance alerts you to create incorrect not functional oligonucleotides before starting the synthesis.

Service

IBA offers free technical assistance provided by nucleic acid chemists and molecular biologists. Take advantage of twenty years of experience in the field of nucleoside-, nucleotide- and oligonucleotide synthesis and application.

Highest Quality - Iso certified!

IBA delivers only highly purified products. We make no compromise in our efforts to reach reproducibly high coupling yields. We have also developed highly sophisticated purification processes to meet the individual needs of each oligonucleotide and modification.

The following points are strictly followed during synthesis of oligonucleotides:

  • Synthesizers from leading manufacturers
  • High quality chemicals from selected suppliers
  • High concentrations of building blocks
  • In-house developed and optimized synthesis cycles
  • Prolonged coupling steps optimized for certain building blocks
  • Purification of oligonucleotides either by HPLC and/or PAGE
  • Two-step purification process for fluorescent probes
  • Exact yield determination by measuring UV-absorption and adjustment of the concentration
  • Routine use of our products for in-house applications like cDNA synthesis, PCR, in vitro transcription, DNA sequencing and gene synthesis
Synthesis scales and typical yields

For an unmodified 20mer with equal base composition (DNA and RNA):

Scale Typical yield Mol. Biol. grade Typical yield after HPLC
0.01 µmol 2-3 nmol 1-2 nmol
0.05 µmol 10-15 nmol 5-8 nmol
0.20 µmol 30-50 nmol 25-40 nmol
1.00 µmol 120-200 nmol 100-150 nmol
10.00 µmol 1,000-1,500 nmol 600-1,000 nmol
15.00 µmol 2,000-3,500 nmol 1,500-3,000 nmol
Storage

Lyophilized oligonucleotides can be stored at 4 °C for several months, and at -20 °C for several years. Once resuspended, please aliquot and store at -20 °C to avoid repeated freeze-thaw cycles. 

For resuspension, spin down the pellet prior to opening the tube. Labeled probes must also be protected from light. If an oligonucleotide is required several times throughout the day, it should be kept at 4 °C.

How to make a stock solution

In order to calculate the buffer volume (in µl) which is to be added in order to get a 100 µM solution, the number of nmoles in the tube should be multiplied by 10, e.g. 9 nmoles oligo > add 90 µl buffer to get a 100 µM solution.

 

Buffer conditions of IBA oligonucleotides

DNA, unmodified: 
sterile water, pH7
DNA with fluorescent label: 
TrisEDTA
DNA with other modification:      
sterile water, pH7
RNA: DEPC-treated water
Hybrids: DEPC-treated water

 

Shipment

For shipments within Germany you may select „lyophilized“ or „in solution“ (standard concentration 0.1 nmol/µl). To all other countries oligonucleotides are delivered lyophilized. Remember to shortly spin down the pellet before opening the tube the first time. The dry pellet may otherwise be lost if not at the bottom of the tube.

 

Production timelines oligonucleotides

Production timelines oligonucleotides

Pricing and delivery information

For our local customers in Göttingen we offer a free-of-charge delivery service directly to your lab!

Our web site special for you! Online orders above EUR 750 [USD 750] are shipped free of charge! For all other shipments, including orders by e-mail, fax or phone, the price table below applies.

 


 Room temperature 

Wet ice

Dry ice

 

USD

EUR

USD

EUR

USD

EUR

Germany

 

7

 

20

 

25

European Union

 

30

 

40

 

85

USA, Hawaii, Canada

59

 

95

 

135

 

Switzerland & Norway

 

37

 

50

 

100

Rest of World*

96

70

136

101

169

125

*Invoices (including shipping cost) for North and South America are issued in USD, all other countries not listed separately above  receive invoices (including shipping cost) in EUR.

 

Purification grades

General information on nucleic acid purification

Modern oligonucleotide synthesis via phosphoramidites is a complex multistep procedure. One elongation cycle consists of the following steps:

  • Deprotection of the 5'-hydroxy group
  • Coupling of the appropriate building block
  • Capping of unreacted 5'-hydroxy groups
  • Oxidation of the phosphittriester group to phosphatetriester

Even if all steps work optimally, a significant amount of incomplete sequences, called trityl-off failure sequences, will accumulate. The amount of these failure sequences depends on the chain length. The figure shows the amount of full length product depending on oligonucleotide length and three different coupling efficiencies (98.0 %, 98.5 %, 99.0 %).

As the oligonucleotide length increases, the amount of full length product decreases. Furthermore, in cases where chain length is greater than 50 bases, the growing chains interact with one another, causing the flow of chemicals through the column to slow down. Trityl-on failure sequences will be present, caused by inefficient detritylation, capping, oxidation or by depurination, resulting in post synthesis strand scissure during ammonia deprotection. In addition to trityl-off and trityl-on failure sequences, the base protecting groups in the form of their corresponding amides will be present within the crude reaction mixture. To fulfill the demand of purity for different applications, IBA offers a broad spectrum of standard and special purification methods.

DNA purification grades

Molecular biology grade purification

Salts, base protecting groups and short failure sequences up to 6 bases are removed efficiently. The amount of full length product depends on chain length and coupling yield. A typical 20mer with an average coupling yield of 98.5 % is composed of 70 % of full length product. This purification is recommended for oligonucleotides with a chain length up to 49 bases for standard molecular biology applications such as PCR, sequencing, etc..

Order Information

Product
Scale
Amounts [oD]*
 Price/oligonucleotide 
Molecular biology grade purification
0.01 µmol
0.5 -1.0
no charge
0.05 µmol
2.0 -4.0
no charge
0.20 µmol
10.0 -20.0
no charge
1.00 µmol
50.0 -100.0
no charge
15.00 µmol
600.0 -1200.0
no charge

 

* delivery amounts for a 20mer with an average base compostion of 5xA, 5xG, 5xC and 5xT

HPLC grade purification

Apart from salts and base protecting groups, the trityl-on HPLC step efficiently removes trityl-off failure sequences. The purity of the product depends on the nature and length of the sequence. IBA recommends this purification for all applications where full-length or high reproducibility are essential, including: cloning, PCR, mutagenesis, probes, modified oligonucleotides and oligonucleotides longer than 40 bases. This method is not able to remove trityl-on failure sequences.

Order Information

Product
Scale
Amount
Cat. no.
Price/oligonucleotide
HPLC grade purification
0.01 µmol
1-2 nmol
5-1020-001
11.00 EUR
0.05 µmol
5-8 nmol
5-1020-002
11.00 EUR
0.20 µmol
25-40 nmol
5-1020-003
12.00 EUR
1.00 µmol
100-150 nmol
5-1020-004
20.00 EUR
10.00 µmol   5-1020-006 147.00 EUR
15.00 µmol
1500-3000 nmol
5-1020-005
210.00 EUR
Double HPLC grade purification

This protocol consists of a trityl-on HPLC purification and a subsequently performed trityl-off HPLC purification. The second step completely removes oligonucleotides with base modifications and partly removes trityl-on failure sequences effectively.

Order Information

Product
Scale
Amount
Cat. no.
Price/oligonucleotide
Double HPLC grade purification
0.01 µmol
0.5-1 nmol
5-1030-001
21.00 EUR
0.05 µmol
2.5-5 nmol
5-1030-002
24.00 EUR
0.20 µmol
10.0-25 nmol
5-1030-003
29.00 EUR
1.00 µmol
50.0-100 nmol
5-1030-004
35.00 EUR
10.00 µmol   5-1030-006 252.00 EUR
15.00 µmol
750.0-1500 nmol
5-1030-005
341.00 EUR
Cell culture grade purification

This unique in-house development is a multi-step purification method for all oligonucleotides used in cell culture. It consists of two HPLC purification steps and one sterile filtration step.

Order Information

Product
Scale
Amount
Cat. no.
Price/oligonucleotide
Cell culture grade purification
0.05 µmol
1-3 nmol
5-1050-002
35.00 EUR
0.20 µmol
5 -15 nmol
5-1050-003
44.00 EUR
1.00 µmol
25 -50 nmol
5-1050-004
53.00 EUR
10.0 µmol   5-1050-006 420,00 EUR
15.0 µmol
500 -1000 nmol
5-1050-005
635.00 EUR
DS grade purification with double HPLC

This method was developed especially for ultra-short oligonucleotides with a chain length between 1 and 7 bases. It consists of an in-house developed 2 x HPLC purification and a desalting step.

Order Information

Product
Scale
Amount
Cat. no.
Price/oligonucleotide
DS grade purification
0.05 µmol
1 -3 nmol
5-1040-002
35.00 EUR
0.20 µmol
5 -15 nmol
5-1040-003
44.00 EUR
1.00 µmol
25-50 nmol
5-1040-004
53.00 EUR
10.00 µmol   5-1040-006 420.00 EUR
15.00 µmol
500 -1000 nmol
5-1040-005
635.00 EUR
Premium PAGE grade

Premium PAGETM is the most powerful method for complete removal of all types of failure sequences and intercalating dyes like TAMRA. Due to the lower recovery rate IBA cannot guarantee delivery amounts.

Recommended for the following products:

  • RNA
  • Oligonucleotides for biophysical application
  • FCS-Spectroscopy 

Order Information

Product
Scale
Cat. no.
Price/oligonucleotide
Premium PAGETM purification
0.05 µmol
5-1060-002
116.00 EUR
0.2 µmol
5-1060-003
142.00 EUR
1.0 µmol
5-1060-004
179.00 EUR
10.0 µmol 5-1060-005 1313.00 EUR
15.0 µmol 5-1060-006 1838.00 EUR
Reverse phase C18 HPLC purification

For oligonucleotides requiring more than standard reverse phase purification, such as double dye labeled oligonucleotides.

Order Information

Product
Scale
Cat. no.
Price/oligonucleotide
Reverse phase C18 HPLC purification
0,05 µmol
5-1022-002
32.00 EUR
0,2 µmol
5-1022-003
42.00 EUR
1 µmol
5-1022-004
47.00 EUR
10 µmol 5-1022-006 368.00 EUR
15 µmol 5-1022-007 525.00 EUR
Documentation

Order Information

Product
Cat. no.
Price/oligonucleotide
Premium PAGETM documentation
5-1070-000
12.00 EUR
HPLC documentation
5-1070-010
12.00 EUR

RNA purification grades

RNA HPLC grade purification

For certain applications RNA HPLC grade purification may be sufficient (but generally PAGE purification is recommended).

Order Information

Product
Scale
Cat. no.
Price/oligonucleotide
RNA HPLC grade purification

0.2 µmol
5-1021-003
47.00 EUR
1 µmol
5-1021-004
79.00 EUR
10 µmol 5-1021-005 578.00 EUR
15 µmol
5-1021-006
798.00 EUR
RNA double HPLC purification

Order Information

Product
Scale
Cat. no.
Price/oligonucleotide
RNA double HPLC purification
0.2 µmol
5-1024-003
69.00 EUR
1 µmol
5-1024-004
81.00 EUR
10 µmol 5-1024-005 609.00 EUR
15 µmol 5-1024-006 830.00 EUR
RNA cell culture grade purification

Order Information

Product
Scale
Cat. no.
Price/oligonucleotide
RNA cell culture grade purification
0.2 µmol
5-1023-003
58.00 EUR
1 µmol
5-1023-004
81.00 EUR
10 µmol 5-1023-005 609.00 EUR
15 µmol 5-1023-006 830.00 EUR
RNA desalting

Order Information

Product
Scale
Cat. no.
Price/oligonucleotide
RNA desalting
1 µmol
5-1041-004
23.00 EUR
10 µmol 5-1041-006 158.00 EUR
15 µmol
5-1041-005
189.00 EUR
Premium PAGE grade

Premium PAGETM is the most powerful method completely removing all types of failure sequences and intercalating dyes like TAMRA. Due to the lower recovery rate IBA cannot guarantee delivery amounts.

Recommended for following products:

  • RNA
  • Oligonucleotides for biophysical application
  • FCS-Spectroscopy 

Order Information

Product
Scale
Cat. no.
Price/oligonucleotide
Premium PAGETM purification
0.05 µmol
5-1060-002
116.00 EUR
0.2 µmol
5-1060-003
142.00 EUR
1.0 µmol
5-1060-004
179.00 EUR
10.0 µmol 5-1060-005 1313.00 EUR
15.0 µmol 5-1060-006 1838.00 EUR
Documentation

Order Information

Product
Cat. no.
Price/oligonucleotide
Premium PAGETM documentation
5-1070-000
12.00 EUR
HPLC documentation
5-1070-010
12.00 EUR