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Paper of the month

This section provides interesting paper and application examples in which IBA products have been successfully applied.

February 2019 - Cryo-EM structure of an envelope protein trimer from HIV-1

Ananthaswamy, N., Fang, Q., AlSalmi, W., Jain, S., Chen, Z., Klose, T., ... & Tovanabutra, S. (2019). A sequestered fusion peptide in the structure of an HIV-1 transmitted founder envelope trimer. Nature Communications10(1), 873. [Link]

January 2019 - AP-MS mapping of protein interactions

Liu, X., Salokas, K., Tamene, F., Jiu, Y., Weldatsadik, R. G., Öhman, T., & Varjosalo, M. (2018). An AP-MS-and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. Nature communications9(1), 1188. [Link]

Dezember 2018 - Immunoaffinity chromatography of lymphocytes

Mohr F., Przibilla S., Leonhardt F., Stemberger C., Dreher S., Müller T. R., Fräßle S. P., Schmidt G. P., Kiene M.-L., Stadler H. & Busch D. H. (2018). Efficient immunoaffinity chromatography of lymphocytes directly from whole blood. Scientific Reports 8, Article number: 16731 [Link

November 2018 - Immobilization on nanoparticles

Becker, A., Leskau, M., Schlingmann-Molina, B. L., Hohmeier, S. C., Alnajjar, S., Escobar, H. M., & Ngezahayo, A. (2018). Functionalization of gold-nanoparticles by the Clostridium perfringens enterotoxin C-terminus for tumor cell ablation using the gold nanoparticle-mediated laser perforation technique. Scientific Reports, 8(1), 14963 [Link]

October 2018 - FRET


Björn Hellenkamp, Sonja Schmid, Olga Doroshenko, [...],Thorsten Hugel (2018) Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study. Nature Methods. 15: (669676) [link]

September 2018 - Membrane protein purification

Membrane protein purification

Lalaurie, C. J., Dufour, V., Meletiou, A., Ratcliffe, S., Harland, A., Wilson, O., ... & Sessions, R. B. (2018). The de novo design of a biocompatible and functional integral membrane protein using minimal sequence complexity. Scientific reports, 8(1), 14564. [LINK]

August 2018 - Protein expression and purification

Protein expression and purification

Wang L, Fan M, Zeng C, Li W, Hu Q, Liu W, Huang X, Li G, Yu F. (2018) Expression and purification of a rapidly degraded protein, TMEM8B-a, in mammalian cell line. Protein Expr Purif. 151: 38-45. [Link]

July 2018 - Protein purification

Protein purification

Essuman K, Summers DW, Sasaki Y, Mao X, Yim AKY, DiAntonio A, Milbrandt J. (2018) TIR Domain Proteins Are an Ancient Family of NAD+ -Consuming Enzymes. Curr Biol. 28(3): 421-430 [Link]

June 2018 - Protein purification

Protein purification

Zhang Y, Ptacin JL, Fischer EC, Aerni HR, Caffaro CE, San Jose K, Feldman AW, Turner CR, Romesberg FE. (2017) A semi-synthetic organism that stores and retrieves increased genetic information. Nature. 551(7682): 644-647. [Link]

May 2018 - Pull Down Assay

Pull-down assay

González M, Brito N, González C. (2017) The Botrytis cinerea elicitor protein BcIEB1 interacts with the tobacco PR5-family protein osmotin and protects the fungus against its antifungal activity. New Phytol. 215(1): 397-410. [Link]

April 2018 - RNA co-immunoprecipitation

RNA co-immunoprecipitation

Chen Z, Mashburn-Warren L, Merritt J, Federle MJ, Kreth J. (2017) Interference of a speB 5' untranslated region partial deletion with mRNA degradation in Streptococcus pyogenes. Mol Oral Microbiol. 32(5): 390-403. [Link]

March 2018 - Protein purification and analysis

Protein purification and analysis (Strep-Tactin® Superflow high capacity, StrepMAB Classic and Strep-Tactin® microplates)

Roman-Sosa, G., Brocchi, E., Schirrmeier, H., Wernike, K. Schelp, C. and Beer M. (2016)
Analysis of the humoral immune response against the envelope glycoprotein Gc of Schmallenberg
virus reveals a domain located at the amino terminus targeted by mAbs with neutralizing activity
Journal of General Virology 97, 571–580; DOI 10.1099/jgv.0.000377 [link]
February 2018 - Membrane protein purification and analysis

Membrane protein purification and analysis (Strep-Tactin® Sepharose and Superflow® high capacity, StrepMAB Classic HRP, Strep-Tactin® Chromeo 488 and Strep-Tactin® microplates)

Hassaine, G., Deluz, C., Grasso, L., Wyss, R., Hovius, R., Stahlberg,H., Tomizaki, T., Desmyter, A.,
Moreau, C., Peclinovska, L., Minniberger, S., Mebarki, L., Li, X.D., Vogel, H., and Nury H. (2017)
Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor
Methods in Molecular Biology, Vol. 1635, DOI 10.1007/978-1-4939-7151-0_8 [Link]


January 2018 - Protein purification and analysis

Protein purification and analysis (Strep-Tactin Resin, StrepMAB antibody)

van Winden, V. J. C., Ummels, R., Piersma, S. R., Jiménez, C. R., Korotkov, K. V., Bitter, W., Houben, E. N. G. (2016)
Mycosins Are Required for the Stabilization of the ESX-1 and ESX-5 Type VII Secretion Membrane Complexes
ASM, Volume 7, 5, e01471-16 [link]