5-4805-001

pLSG-IBA5 vector

Insect expression vector encoding an N-terminal Strep-tag®II

€219.00*

Content
The pLSG-IBA5 vector is designed for high-level expression of target proteins with an N-terminal Strep-tag®II in insect cells. The gene transfer into the polyhedrin gene locus of AcMNPV DNA is achieved by homologous recombination and the vector carries a polyhedrin promoter. Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or with circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild-type virus to great extent. For selection and propagation in E. coli, the vector carries an ampicillin resistance and ColE1 origin of replication (pUC). Please note that cloning into pLSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pLSG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

Affinity Tag N'-terminal: Strep-tag®II
Cloning Method: Direct cloning using restriction enzyme Esp3I
Concentration: 250 ng/µl
Expression Host: Insect cells
Form: Suspension in TE buffer
Possible Application: Vector for recombinant expression in insect cells
Promoter: Polyhedrin Promoter
Resistance: Ampicillin
Sequence: See Documents
Size: 6194 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Competent E. coli TOP10
Chemically competent cells for plasmid propagation
pENTRY-IBA51 vector
Entry vector for generation of StarGate donor vectors

Related Products

pLSG-IBA3 vector
Insect expression vector encoding a C-terminal Strep-tag®II