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Receptor-specific isolation of lymphocytes with reversible Fab Streptamers®

Magnetic Fab Streptamers® are directed against cell surface markers including those of T cells (CD3, CD4, CD8), memory T cells (CD8, CD62L, CD45RA-), regulatory T cells (CD4, CD25, CD45RA) and B cells (CD19). They consist of two components:

1) Fab-Streps (= Strep-tagged Fab fragments) which recognize the surface markers

2) Magnetic beads (= Strep-Tactin® magnetic beads) which reversibly bind the Fab-Streps

For cell isolation with Fab-Streps we offer microbeads (Strep-Tactin® magnetic microbeads) and our hand-held StrepMan permanent magnet (see below).

Cell Isolation with Magnetic Microbeads (for permanent magnets)

1) Fab-Streps (Strep-tagged Fab fragments) are multimerized with Strep-Tactin® attached to a magnetic bead to generate multimers with high binding avidity (Fig.1).

Strep-tagged Fab fragments
Fig.1: Fab Streptamer® Magnetic Microbead

2) Selection of the target cells is performed with our StrepMan permanent magnet. We recommend a horizontal position for separation of the magnetically labeled cells (top). For removal of the magnetic Streptamers from the isolated cells with biotin we recommend an upright position of the StrepMan magnet (bottom). Cells are left phenotypically and functionally indistinguishable from untreated cells.

Reversible manual cell isolation with Streptamer Magnetic Microbeads
StrepMan magnet for Streptamer® Cell Isolation with Magnetic Microbeads

Fig.2: StrepMan magnet for Streptamer® cell isolation with Strep-Tactin® magnetic microbeads