Protein A

As the developer of high quality tools for recombinant protein production, we have extended our product portfolio by Protein A Agarose affinity resins.

Protein A is commonly used for the affinity purification of IgG class antibodies. It binds to immunoglobulins through interaction with the IgG heavy chain within the Fc region and purifies all antibodies of the target class without regard to antigen specificity. Antibodies from serum (polyclonal antibodies), ascites or cell culture supernatant of hybridoma cell lines (monoclonal antibodies) can thus be easily isolated.

IBAs' Protein A provides

  • High NaOH stability (CIP)
  • High binding capacity
  • Cost effective products

Now 25% off introductory price*!

*Discount is valid until March 31st 2019. Not valid in combination with other IBA discounts. Distributors and IBA staff excluded.

Product shop

Product Contents Cat.no Price   Order
Protein A Agarose 50% suspension; 1 ml 1 ml 6-2010-001 USD 51.00  
6-2010-001

25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ...

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Protein A Agarose 50% suspension; 3 ml 3 ml 6-2010-002 USD 116.00  
 
6-2010-002

25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ...

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Protein A Agarose 50% suspension; 10 ml 10 ml 6-2010-005 USD 298.00  
 
6-2010-005

25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ...

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Protein A Agarose 50% suspension; 50 ml 50 ml 6-2010-025 USD 1,182.00  
 
6-2010-025

25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ...

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Protein A Agarose 50% suspension; 200 ml 200 ml 6-2010-100 USD 3,782.00  
 
6-2010-100

25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ...

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Gravity flow Protein A Agarose column; 0,5 ml 1 x 0,5 ml 6-2015-001 USD 38.00  
6-2015-001

25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ...

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Protein A Agarose cartridge; 1 ml 1 x 1 ml 6-2021-001 USD 181.00
6-2021-001

25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ...

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Protein A Agarose cartridge; 5 ml 1 x 5 ml 6-2022-001 USD 714.00
6-2022-001

25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ...

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Technical specifications

Dynamic binding capacity (DBC 10%) of IBA Protein A determined with a  monoclonal antibody.

DBC of Protein A remains stable after treatment with 0.5 M NaOH over~100 cycles. 1 cycle = 15 min.

Data

 

Capacity* (DBC 10%) ≥70 mg human IgG/mL resin at residence time 6 min
≥50 mg human IgG/mL resin at residence time 2.4 min
≥25 mg human IgG/mL resin at residence time 1 min
Flow rate 0.16 - 1 mL/min (1 mL cartridge)
0.83 - 5 mL/min (5 mL cartridge)
250 cm/h (50% suspension)
CIP (0.5 M NaOH 100 Cycles) No significant change in performance after 100 cycles with 0.5 M NaOH (1 cycle = 15 min contact time) use at room temperature
Particle size 45 µm 

*Dynamic binding capacity (DBC 10%) was determined with 1 mg/ml human monoclonal IgG1 using a 1 mL column at 1, 2.4 and 6 minutes residence time.

 

Protein A does not bind to all IgG's equivalently and binding varies among species. Even within a species, Protein A interacts with some subclasses of IgG and not others.