As the developer of high quality tools for recombinant protein production, we have extended our product portfolio by Protein A Agarose affinity resins.
Protein A is commonly used for the affinity purification of IgG class antibodies. It binds to immunoglobulins through interaction with the IgG heavy chain within the Fc region and purifies all antibodies of the target class without regard to antigen specificity. Antibodies from serum (polyclonal antibodies), ascites or cell culture supernatant of hybridoma cell lines (monoclonal antibodies) can thus be easily isolated.
IBAs' Protein A provides
- High NaOH stability (CIP 0.5 M NaOH 100 Cycles)
- ≥70 mg/ml binding capacity
- Cost effective products
Now 25% off introductory price*!
*Discount is valid until March 31st 2019. Not valid in combination with other IBA discounts. Distributors and IBA staff excluded.
Dynamic binding capacity (DBC 10%) of IBA Protein A determined with a monoclonal antibody.
DBC of Protein A remains stable after treatment with 0.5 M NaOH over~100 cycles. 1 cycle = 15 min.
|Capacity* (DBC 10%)||≥70 mg hu IgG/mL resin at residence time 6 min
≥50 mg hu IgG/mL resin at residence time 2.4 min
≥25 mg hu IgG/mL resin at residence time 1 min
|Flow rate||0.16 - 1 mL/min (1 mL cartridge)
0.83 - 5 mL/min (5 mL cartridge)
250 cm/h (50% suspension)
|CIP (0.5 M NaOH 100 Cycles)||No significant change in performance after 100 cycles with 0.5 M NaOH (1 cycle = 15 min contact time) use at room temperature|
|Particle size||45 µm|
*Dynamic binding capacity (DBC 10%) was determined with 1 mg/ml monoclonal hu IgG1 using a 1 mL column at 1, 2.4 and 6 minutes residence time.
Protein A does not bind to all IgG's equivalently and binding varies among species. Even within a species, Protein A interacts with some subclasses of IgG and not others.