Protein A

As the developer of high quality tools for recombinant protein production, we have extended our product portfolio by Protein A Agarose affinity resins.

Protein A is commonly used for the affinity purification of IgG class antibodies. It binds to immunoglobulins through interaction with the IgG heavy chain within the Fc region and purifies all antibodies of the target class without regard to antigen specificity. Antibodies from serum (polyclonal antibodies), ascites or cell culture supernatant of hybridoma cell lines (monoclonal antibodies) can thus be easily isolated.

IBAs' Protein A provides

  • High NaOH stability (CIP 0.5 M NaOH 100 Cycles)
  • ≥70 mg/ml dynamic binding capacity (DBC 10%)
  • Cost effective products

Order your free sample here!

Product shop

Product Contents Cat.no Price   Order
Protein A Agarose 50% suspension; 1 ml 1 ml 6-2010-001 USD 51.00  
6-2010-001

Protein A Agarose 50% (v/v) suspension is developed for purification of monoclonal- and polyclonal antibodies using affinity chromatography. ...

Further details, manuals, data sheets, related products and more
Protein A Agarose 50% suspension; 3 ml 3 ml 6-2010-002 USD 116.00  
6-2010-002

Protein A Agarose 50% (v/v) suspension is developed for purification of monoclonal- and polyclonal antibodies using affinity chromatography. ...

Further details, manuals, data sheets, related products and more
Protein A Agarose 50% suspension; 10 ml 10 ml 6-2010-005 USD 298.00  
6-2010-005

Protein A Agarose 50% (v/v) suspension is developed for purification of monoclonal- and polyclonal antibodies using affinity chromatography. ...

Further details, manuals, data sheets, related products and more
Protein A Agarose 50% suspension; 50 ml 50 ml 6-2010-025 USD 1,182.00  
6-2010-025

Protein A Agarose 50% (v/v) suspension is developed for purification of monoclonal- and polyclonal antibodies using affinity chromatography. ...

Further details, manuals, data sheets, related products and more
Protein A Agarose 50% suspension; 200 ml 200 ml 6-2010-100 USD 3,782.00  
6-2010-100

Protein A Agarose 50% (v/v) suspension is developed for purification of monoclonal- and polyclonal antibodies using affinity chromatography. ...

Further details, manuals, data sheets, related products and more
Gravity flow Protein A Agarose column; 0.5 ml 6-2015-001 USD 38.00  
6-2015-001

Gravity flow Protein A Agarose column for purification of monoclonal- and polyclonal antibodies using affinity chromatography. ...

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Protein A Agarose cartridge 1 ml 6-2021-001 USD 181.00
6-2021-001

Protein A Agarose cartridge for purification of monoclonal- and polyclonal antibodies with HPLC or FPLC ...

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Protein A Agarose cartridge 5 ml 6-2022-001 USD 714.00
6-2022-001

Protein A Agarose cartridge for purification of monoclonal- and polyclonal antibodies with HPLC or FPLC ...

Further details, manuals, data sheets, related products and more

Technical specifications

Dynamic binding capacity (DBC 10%) of IBA Protein A determined with a  monoclonal antibody.

DBC of Protein A remains stable after treatment with 0.5 M NaOH over~100 cycles. 1 cycle = 15 min.

Data

 

Capacity* (DBC 10%) ≥70 mg hu IgG/mL resin at residence time 6 min
≥50 mg hu IgG/mL resin at residence time 2.4 min
≥25 mg hu IgG/mL resin at residence time 1 min
Flow rate 0.16 - 1 mL/min (1 mL cartridge)
0.83 - 5 mL/min (5 mL cartridge)
250 cm/h (50% suspension)
CIP (0.5 M NaOH 100 Cycles) No significant change in performance after 100 cycles with 0.5 M NaOH (1 cycle = 15 min contact time) use at room temperature
Particle size 45 µm 

*Dynamic binding capacity (DBC 10%) was determined with 1 mg/ml monoclonal hu IgG1 using a 1 mL column at 1, 2.4 and 6 minutes residence time.

 

Protein A does not bind to all IgG's equivalently and binding varies among species. Even within a species, Protein A interacts with some subclasses of IgG and not others.