Strep-Tactin® conjugate

Strep-Tactin® conjugates for detection of Strep-tag®II and Twin-Strep-tag® fusion proteins


The streptavidin variant Strep-Tactin® is available as conjugate with horseradish peroxidase (HRP) or with alkaline phosphatase (AP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag®II or Twin-Strep-tag® fusion proteins transferred to a blotting membrane. Both conjugates are suitable for chromogenic reaction, whereas Strep-Tactin® HRP is applicable for chemiluminescence reaction as well.
Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins, e.g., the biotin carboxyl carrier protein (BCCP, 22 kDa ) of E. coli. Biotin Blocking Buffer is recommended for blocking of such biotinylated proteins on western blots prior to detection of Strep-tag®II or Twin-Strep-tag® fusion proteins with Strep-Tactin® HRP to prevent the detection of false positive bands. For this purpose, Biotin Blocking Buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins.


Concentration: Strep-Tactin® HRP: 0.95-1.05 mg/ml, Strep-Tactin® AP: 0.175-0.185 mg/ml
Conjugate: AP, HRP
Form: Suspension in PBS
Possible Application: Western blot, detection in microplates
Specificity: Twin-Strep-Tag®; Strep-tag®II; cross reactivity: biotin

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature


GFP-Strep-tag control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
Strep-tag®II peptide
Synthetic peptide recognized by Strep-Tactin® and Strep-Tactin®XT
Biotin Blocking Buffer
Efficient blocking buffer for biotinylated proteins in western blot analyses

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