Application examples

Strep-Tactin® HRP Western Blot
Strep-tag protein detection using Strep-Tactin HRP conjugate in western blot

Direct detection of recombinant Strep-tag®II GFP in a Western blot using Strep-Tactin® HRP conjugate.

Strep-Tactin® HRP dot blot
Detection of Strep-tag in dot blot by using Strep-Tactin HRP conjugate

Direct detection of recombinant Strep-tag®II proteins GFP (MW 28 kDa) and E. coli alkaline phosphatase (monomer 48.5 kDa) in a dot blot usinf Strep-Tactin® HRP conjugate.

Strep-Tactin® AP western blot
Strep-Tactin AP conjugate for detection of Strep-tag II in western blot

Direct detection of recombinant Strep-tag®II GFP in a Western blot using Strep-Tactin® AP conjugate.

Strep-Tactin® AP dot blot
Direct detection of proteins containing Strep-tag by using Strep-Tactin AP conjugate in dot blot

Direct detection of recombinant Strep-tag®II proteins GFP (MW 28 kDa) and E. coli alkaline phosphatase (monomer 48.5 kDa) in a dot blot usinf Strep-Tactin® AP conjugate.

Immunofluorescence
Detection of recombinant proteins fused to Strep-tag via immunofluorescence

Type-I transmembrane protein (extracellular domain, N-terminal Strep-tag®) stained with Strep-Tactin® Chromeo 546 1:100 in insect cells. Fixation: 4 % Formaldehyd in PBS, 20 minutes RT
Kindly provided by: Dr. Michael Krahn, Abteilung Stammzellbiologie, GZMB Göttingen