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Application examples for Detection with Strep-Tactin® Conjugates

Strep-Tactin® HRP Western Blot
Strep-tag® protein detection using Strep-Tactin® HRP conjugate in western blot

Direct detection of recombinant Strep-tag®II GFP in a Western blot using Strep-Tactin® HRP conjugate.

Strep-Tactin® HRP dot blot
Detection of Strep-tag® in dot blot by using Strep-Tactin® HRP conjugate

Direct detection of recombinant Strep-tag®II proteins GFP (MW 28 kDa) and E. coli alkaline phosphatase (monomer 48.5 kDa) in a dot blot usinf Strep-Tactin® HRP conjugate.

Strep-Tactin® AP western blot
Strep-Tactin® AP conjugate for detection of Strep-tag®II in western blot

Direct detection of recombinant Strep-tag®II GFP in a Western blot using Strep-Tactin® AP conjugate.

Strep-Tactin® AP dot blot
Direct detection of proteins containing Strep-tag®II by using Strep-Tactin® AP conjugate in dot blot

Direct detection of recombinant Strep-tag®II proteins GFP (MW 28 kDa) and E. coli alkaline phosphatase (monomer 48.5 kDa) in a dot blot usinf Strep-Tactin® AP conjugate.

FACS with Strep-Tactin® PE or Strep-Tactin® APC

- Wash 5x106 pre-cooled cells with 10 ml of a biotin-free physiological wash buffer in a 15 ml reaction tube to remove potentially interfering  ingredients (e.g. biotin).

- Resuspend the cells in 50 μl wash buffer and transfer cells to a reaction vessel suitable for staining, e.g. a 96-well round bottom microtiter plate.

- Add 5 µl Strep-Tactin®PE (or Strep-Tactin®APC) to the cells and mix thoroughly by gentle pipetting.

- Incubate for 20 minutes at 4°C in the dark.

- Wash cells three times (400xg, 2 min) in 200 μl wash buffer.

- Cells are ready for FACS

Note: 50 μl conjugate are good for staining of approximately 5x107to 1x108 cells. The staining intensity depends on the concentration of tagged protein and in case of unsatisfying results the concentration of conjugate has to be adjusted.