Strep-Tactin® PE or APC
Fluorophore-conjugated Strep-Tactin® for cell staining
Strep-Tactin® conjugated to either R-Phycoerythrin (PE) or Allophycocyanin (APC) is suitable for staining or isolation of antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®) based on our Strep-tag® technology. To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin®. Strep-Tactin® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting fluorescent complexes can be used to stain cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents preserves the cells’ full biological activity.
|Form:||Suspension in DPBS|
|Possible Application:||Fluorescent cell staining & sorting|
|Recommended Usage:||1 µl per 2 x 10^6 (MHC I-Streps) or 5 x 10^6 (Fab-Streps) total cells|
|Storage:||2-8 °C; Do not freeze!|
|Stability:||12 months after shipping|