2-1202-051

Strep-Tactin® Sepharose® column

Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

€218.00*

Content
Strep-Tactin® Sepharose® columns contains 4% agarose coupled with the streptavidin variant Strep-Tactin® and are applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins via gravity flow. Due to the low concentrated agarose, it is particularly suitable for large proteins, especially if the affinity to Strep-Tactin®XT 4Flow® is too high. The wide-meshed polymer allows for the simple attachment of larger proteins to the stationary phase.
To reduce the waiting period during sample and buffer application, the use of the application aid WET FRED is recommended.

Specifications

Bead Size: 45 - 165 µm
Biotin Binding Capacity: > 300 nmol/ml resin
Exclusion Limit: 3 x 10^7
Form: Pre-packed gravity flow column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-Tag®; Strep-tag®II; cross reactivity: biotin
Strep-tag®II Binding Capacity: 3 mg/ml
Support: 4% agarose

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

WET FRED Set
Application aid for large volumes of lysates or culture supernatants
BioLock
Biotin blocking solution for lysates and culture supernatants
Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set
10x Buffer W
Strep-Tactin® and Strep-Tactin®XT wash buffer
10x Buffer E
Strep-Tactin® elution buffer with desthiobiotin
10x Buffer R
Strep-Tactin® regeneration buffer with HABA
Desthiobiotin
Desthiobiotin powder for preparation of Strep-Tactin® elution buffer
Avidin
Efficient blocking of biotin and biotinlyated proteins

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