2-1201-002

Strep-Tactin® Sepharose® resin

50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

€98.00*

Content
Strep-Tactin® Sepharose® is a 4% agarose coupled with the streptavidin variant Strep-Tactin® and applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. Due to the low concentrated agarose, it is particularly suitable for large proteins, especially if the affinity to Strep-Tactin®XT 4Flow® is too high. The wide-meshed polymer allows for the simple attachment of larger proteins to the stationary phase. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension is necessary. Please note that Strep-Tactin® Sepharose® is not compatible with HPLC/FPLC due to the low pressure stability.

Specifications

Bead Size: 45 - 165 µm
Biotin Binding Capacity: > 300 nmol/ml resin
Exclusion Limit: 3 x 10^7
Form: 50% suspension
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-Tag®; Strep-tag®II; cross reactivity: biotin
Strep-tag®II Binding Capacity: 3 mg/ml
Support: 4% agarose

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

BioLock
Biotin blocking solution for lysates and culture supernatants
Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set
10x Buffer W
Strep-Tactin® and Strep-Tactin®XT wash buffer
10x Buffer E
Strep-Tactin® elution buffer with desthiobiotin
10x Buffer R
Strep-Tactin® regeneration buffer with HABA
Avidin
Efficient blocking of biotin and biotinlyated proteins
Desthiobiotin
Desthiobiotin powder for preparation of Strep-Tactin® elution buffer

Related Products

Strep-Tactin® Sepharose® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
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Strep-Tactin® Superflow® high capacity cartridge
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Strep-Tactin® MacroPrep® resin
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