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Strep-Tactin® gravity flow columns

Prepacked chromatography columns for gravity flow purification of Strep-tag fusion proteins are availble with different resin supports:

Strep-Tactin^® Sepharose^®
Strep-Tactin^® Superflow^®
Strep-Tactin^® Superflow^® high capacity

They are provided in different sizes (0.2 ml; 1 ml; 5 ml and 10 ml).

When to use which resin?

Sepharose^®	Economic alternative to Superflow^® Agarose support with good flow properties For gravity flow chromatography only (not pressure stable)
Superflow^®	Our all-rounder and the resin to start with for large proteins and protein:protein interaction analysis Agarose support with low unspecific binding properties For increased flow rates Low pressure stable (FPLC/HPLC/Äkta applications)
Superflow^® high capacity (HC)	The capacity of Superflow^® is not sufficient or the binding affinity to Twin-/Strep-tag^® fusion proteins needs to be slightly improved! 3-5 fold higher binding capacity as Superflow®
MacroPrep^®	The alternative if Sepharose^® and Superflow^® lead to non-specific binding of protein (e.g. Chlorophyll from plant extracts) Synthetic support with low non-specific binding properties Highly pressure stable (FPLC/HPLC /Äkta applications) Only available as 50% suspension - not available as ready-to-use gravity flow column

Please use filters "Category" and "Subcategory" to quickly select your product from the list below:

Product	Contents	Cat.no	Price	Order
WET FRED For 5-10ml Gravity flow columns	1 set	2-0910-001	USD 129.00	Quantity:
WET FRED - sample applicator for 5 ml and 10 ml gravity flow columns. ... Further details, manuals, data sheets, related products and more
WET FRED For 1 ml Gravity flow columns	1 set	2-0911-001	USD 114.00	Quantity:
WET FRED - applicator for 1 ml gravity flow columns. ... Further details, manuals, data sheets, related products and more
10x Buffer E; Strep-Tactin® Elution Buffer with Desthiobiotin	25 ml	2-1000-025	USD 64.00	Quantity:
Buffer E is a Strep-Tactin® Elution Buffer with Desthiobiotin (10x concentrated) and used for elution of Strep-tag®II and Twin-Strep-tag® proteins from the biotin-binding site of Strep-Tactin® resins in a competitive manner resulting in a mild elution. ... Further details, manuals, data sheets, related products and more
Strep-Tactin® Purification Buffer Set	1 Set	2-1002-001	USD 90.00	Quantity:
Strep-Tactin® Purification Buffer Set is a convenient buffer set for the purification of recombinant Strep-tag®II and Twin-Strep-tag®II fusion proteins on immobilized Strep-Tactin® and its regeneration. ... Further details, manuals, data sheets, related products and more
10x Buffer R; Strep-Tactin® Regeneration Buffer with HABA;	100 ml	2-1002-100	USD 52.00	Quantity:
Buffer R is a Strep-Tactin® Regeneration Buffer with HABA for the regeneration of immobilized Strep-Tactin®. HABA (2-[4 -hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin (present in Buffer E: Strep-Tactin® Elution Buffer with Desthiobiotin) at the biotin-binding site of Strep-Tactin®. The yellow HABA solution turns red upon binding to Strep-Tactin® clearly indicating the regeneration process and activity status of the column. ... Further details, manuals, data sheets, related products and more
10x Buffer W; Strep-Tactin®/Strep-Tactin®XT Wash Buffer	100 ml	2-1003-100	USD 44.00	Quantity:
Strep-Tactin®/Strep-Tactin®XT Wash Buffer is used for the removal of unbound molecules from immobilized Strep-Tactin® and Strep-Tactin®XT during Strep-tag® protein purification. Further details, manuals, data sheets, related products and more
GFP-Strep-tag control protein	500 µg of a 28 kDa protein	2-1006-005	USD 112.00	Quantity:
GFP-Strep-tag®II control protein for test purifications with Strep-Tactin® and Strep-Tactin® XT.The Strep-tag®II is fused to the C-terminus of the green fluorescent protein (GFP). Further details, manuals, data sheets, related products and more
GFP-Twin-Strep-tag control protein	500 µg of a 30 kDa protein	2-1007-005	USD 112.00	Quantity:
GFP-Twin-Strep-tag® control protein for test purifications with Strep-Tactin® and Strep-Tactin® XT.The Twin-Strep-tag® is fused to the C-terminus of the green fluorescent protein (GFP). Further details, manuals, data sheets, related products and more
Gravity flow Strep-Tactin® Sepharose® column	1 x 1 ml	2-1202-001	USD 90.00	Quantity:
Gravity flow Strep-Tactin® Sepharose® column for one-step purification of recombinant Strep-tag® proteins. ... Further details, manuals, data sheets, related products and more
Gravity flow Strep-Tactin® Sepharose® column	1 x 5 ml	2-1202-051	USD 266.00	Quantity:
Gravity flow Strep-Tactin® Sepharose® column for one-step purification of recombinant Strep-tag® proteins. ... Further details, manuals, data sheets, related products and more
Gravity flow Strep-Tactin® Sepharose® column	1 x 10 ml	2-1202-101	USD 488.00	Quantity:
Gravity flow Strep-Tactin® Sepharose® column for one-step purification of recombinant Strep-tag® proteins. ... Further details, manuals, data sheets, related products and more
Gravity flow Strep-Tactin® Sepharose® mini-column	5 x 0.2 ml	2-1202-550	USD 216.00	Quantity:
Gravity flow Strep-Tactin® Sepharose® column for one-step purification of recombinant Strep-tag® proteins. ... Further details, manuals, data sheets, related products and more