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Strep-Tactin® gravity flow columns

Prepacked chromatography columns for gravity flow purification of Strep-tag fusion proteins are availble with different resin supports:

  • Strep-Tactin® Sepharose®
  • Strep-Tactin® Superflow®
  • Strep-Tactin® Superflow® high capacity
  • Strep-Tactin® Macroprep®

They are provided in different sizes (0.2 ml; 1 ml; 5 ml and 10 ml).

When to use which resin?
Sepharose®
  • Economic alternative to Superflow®
  • Agarose support with good flow properties
  • For gravity flow chromatography only (not pressure stable)

Superflow®

  • Our all-rounder and the resin to start with for large proteins and protein:protein interaction analysis
  • Agarose support with low unspecific binding properties
  • For increased flow rates
  • Low pressure stable (FPLC/HPLC/Äkta applications)

Superflow® high capacity (HC)

  • The capacity of Superflow® is not sufficient or the binding affinity to Twin-/Strep-tag® fusion
  • proteins needs to be slightly improved!
  • 3-5 fold higher binding capacity as Superflow®
MacroPrep®
  • The alternative if Sepharose® and Superflow® lead to non-specific binding of protein (e.g. Chlorophyll from plant extracts)
  • Synthetic support with low non-specific binding propertie
  • Highly pressure stable (FPLC/HPLC /Äkta applications)

 

Please use filters "Category" and "Subcategory" to quickly select your product from the list below:


Product Contents Cat.no Price   Order
WET FRED For 5-10ml Gravity flow columns 1 set 2-0910-001 USD 125.00  
2-0910-001

WET FRED - sample applicator for 5 ml and 10 ml gravity flow columns. ...

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WET FRED For 1 ml Gravity flow columns 1 set 2-0911-001 USD 111.00  
2-0911-001

WET FRED - applicator for 1 ml gravity flow columns. ...

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10x Buffer E; Strep-Tactin® Elution Buffer with Desthiobiotin 25 ml 2-1000-025 USD 62.00  
2-1000-025

Buffer E is a Strep-Tactin® Elution Buffer with Desthiobiotin (10x concentrated) and used for elution of Strep-tag®II and Twin-Strep-tag® proteins from the biotin-binding site of Strep-Tactin® resins in a competitive manner resulting in a mild elution. ...

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Strep-Tactin® Purification Buffer Set 1 Set 2-1002-001 USD 87.00  
2-1002-001

Strep-Tactin® Purification Buffer Set is a convenient buffer set for the purification of recombinant Strep-tag®II and Twin-Strep-tag®II fusion proteins on immobilized Strep-Tactin® and its regeneration. ...

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10x Buffer R; Strep-Tactin® Regeneration Buffer with HABA; 100 ml 2-1002-100 USD 50.00  
2-1002-100

Buffer R is a Strep-Tactin® Regeneration Buffer with HABA for the regeneration of immobilized Strep-Tactin®. HABA (2-[4 -hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin (present in Buffer E: Strep-Tactin® Elution Buffer with Desthiobiotin) at the biotin-binding site of Strep-Tactin®. The yellow HABA solution turns red upon binding to Strep-Tactin® clearly indicating the regeneration process and activity status of the column. ...

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10x Buffer W; Strep-Tactin®/Strep-Tactin®XT Wash Buffer 100 ml 2-1003-100 USD 43.00  
2-1003-100

Strep-Tactin®/Strep-Tactin®XT Wash Buffer is used for the removal of unbound molecules from immobilized Strep-Tactin® and Strep-Tactin®XT during Strep-tag® protein purification.

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GFP-Strep-tag control protein 500 µg of a 28 kDa protein 2-1006-005 USD 109.00  
GFP-Twin-Strep-tag control protein 500 µg of a 30 kDa protein 2-1007-005 USD 109.00  
Gravity flow Strep-Tactin® Sepharose® column 1 x 1 ml 2-1202-001 USD 87.00
2-1202-001

Gravity flow Strep-Tactin® Sepharose® column for one-step purification of recombinant Strep-tag® proteins. ...

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Gravity flow Strep-Tactin® Sepharose® column 1 x 5 ml 2-1202-051 USD 258.00
2-1202-051

Gravity flow Strep-Tactin® Sepharose® column for one-step purification of recombinant Strep-tag® proteins. ...

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Gravity flow Strep-Tactin® Sepharose® column 1 x 10 ml 2-1202-101 USD 474.00  
2-1202-101

Gravity flow Strep-Tactin® Sepharose® column for one-step purification of recombinant Strep-tag® proteins. ...

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Gravity flow Strep-Tactin® Sepharose® mini-column 5 x 0.2 ml 2-1202-550 USD 210.00  
2-1202-550

Gravity flow Strep-Tactin® Sepharose® column for one-step purification of recombinant Strep-tag® proteins. ...

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