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Specifications

Specifications of Strep-Tactin® coated microplates

Specifications of Strep-Tactin® coated microplates

Strep-tag® fusion protein binding capacity 100 ng Strep-tag® II fusion protein/well
Intra- and inter-assay variance (cv) < 1.6 % and < 3.0 %, respectively 
Max. volume per well  300 µl 
Optimal pH  8.0 
Stability > 1 year at 4 °C
Storage  4 °C 
Shipment RT

 

Specifications of StrepMAB-Immo

Specifications of StrepMAB-Immo

Host animal/Subclass
Mouse/IgG1
Specifity
Nearly irreversible binding of C- or N-terminally fused Strep-tag® II, which is N-terminally extended by "SerAla" ** like recombinant protein - SA-WSHPQFEK or SA-WSHPQFEK - recombinant protein. Proteins containing Strep-tag® II with
N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far.
Form
binding capacity
lyophilized from NH4HCO3 with 10 mg sucrose per mg antibody
(determined for bacterial alkaline phosphatase)
Stability
24 months after shipment
Storage
-20 °C
Shipment
RT
**pASK-/pEXPR- and pPR-IBA vectors with N-term One-/Strep-tag® II do NOT have "SerAla"-Linker but the
StarGate® vectors (carrying Strep-tag® II and One-STrEP-tag) are equipped with the SA-Linker.