The MacroPrep® resin exhibits non-specific binding properties differing from those of Sepharose® or Superflow® and can, thus, be recommended in cases where suboptimal results are obtained with Sepharose® or Superflow®, and vice versa (see applications).
MacroPrep® is available in a multitude of formats, click here for all available column formats.
For purification of Strep-tag® II proteins by gravity flow or under low pressure
- Strep-Tactin® MacroPrep® is available as pre-packed gravity flow columns or as H-PR cartridges (for chromatography workstations with 10-32 connections).
Strep-Tactin® Spin columns - for rapid parallel purification of Strep-tag® proteins
- up to 150 µg Strep-tag® protein in less than 10 minutes
- ready-to-use spin columns
- fast parallel processing of multiple samples
- highly cost-effective!
Gravity flow StrepMAB-Classic MacroPrep® Column
An antibody-based option for Strep-tag® protein purification
This new antibody-based purification column with StrepMAB-Classic immobilized to MacroPrep® provides an alternative for purification of Strep-tag® proteins. Using this column as second step following purification via Strep-Tactin®, protein purity can be increased to over 99% while only one tag – the Strep-tag® – is used.
In protein:protein interaction analysis, e.g. this column is used in the so-called One-TAP system, were it enables a second, completely independent purification step with respect to resin (MacroPrep® vs Superflow®) and affinity receptor (mAB vs Strep-Tactin®).