Product Specifications

Avidin Specifications
Description
Avidin is a tetrameric biotin binding protein (KD for biotin > 10-14) from egg white which does not bind to the Strep-tag.
Form
white powder
Activity
binding of >45 nmol biotin/mg avidin (determined by HABA dye binding method)
Stability
12 months after shipping
Storage
-20°C, solutions in dilute buffers are stable at +4°C
Shipment
RT
D-Biotin Specifications
Description
Vitamin H; (+)-Biotin
Form
white powder
FW
244.3, C10H16N2O3S
Stability
12 months after shipping
   
Storage
2 - 8 °C
Shipment
RT

Please note that after elution with Biotin the Strep-Tactin resin cannot be regenerated!

Streptavidin Specifications
Form
Lyophilized in 10 mM potassium phosphate buffer pH 6.5
MW per tetramer
~53 000
Extinction coefficient per subunit
ε280 = 41326 M-1 cm-1
Specific activity
> 17 U/mg (one unit binds 1 µg D-biotin)
Purity
> 95 % as estimated by SDS-PAGE
Proteolytic activity
< 10-3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0)
Reconstitution
Dissolve with water
Storage (lyophilized)
-20 °C
Stability (lyophilized)
2 years after shipping
Storage (dissolved)
4 °C; for longer storage in dissolved form add 1 mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter
Shipment
Room temperature