MEXi product list
Expression of recombinant proteins using MEXi fits perfectly in the Strep-tag® platform IBA provides. This starts by using the recommended pDSG expression vectors, which are compatible with the StarGate expression system, purification with Strep-Tactin® or Strep-Tactin®XT resins and finally confirmation of the expressed protein with Western Blot detection and eventually assay applications.
When secreted target protein will be purified it is important to inactivate the biotin, which is present in nearly all culture media. For this use BioLock:
For purification from large culture volumes use IBA's WET FRED:
| Product | Contents | Cat.no | Price | Order | |||
|---|---|---|---|---|---|---|---|
| BioLock Biotin blocking solution | 50 ml | 2-0205-050 | USD 180.00 | ||||
 Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media. ... |
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| BioLock Biotin blocking solution | 250 ml | 2-0205-250 | USD 725.00 | ||||
 Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media. ... |
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| MEXi-293E cells | 1.5 ml | 2-6001-001 | USD 450.00 | ||||
 MEXi-293E cells are human embryonic kidney (HEK) cells derived from the 293 cell line and used for propagation of plasmids and expression of recombinant proteins in mammalian cells. The cell line expresses the EBNA-1 protein for episomal replication of oriP-harboring plasmids. The cell line is adapted for growth in suspension and for use with the MEXi mammalian expression system. ... |
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| MEXi-CM culture medium | 1000 ml | 2-6010-010 | USD 106.00 | ||||
 MEXi-CM culture medium is a serum free, chemically defined medium especially developed for growth of high-density MEXi-293E cells. It is part of the MEXi mammalian expression system. ... |
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| MEXi-TM transfection medium | 1000 ml | 2-6011-010 | USD 106.00 | ||||
 MEXi-TM transfection medium is a serum free, chemically defined medium especially developed for transient transfection and growth of MEXi HEK293E cells. It is part of the MEXi mammalian expression system. ... |
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| StarGate Acceptor Vector pDSG-IBAwt1 | 5µg | 5-5200-001 | USD 260.00 | ||||
 StarGate Acceptor Vector designed for high-level stable transient expression in most mammalian hosts containing the following elements: ... |
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| StarGate Acceptor Vector pDSG-IBAwt2 | 5µg | 5-5201-001 | USD 260.00 | ||||
 StarGate Acceptor Vector designed for high-level stable transient expression in most mammalian hosts containing the following elements: ... |
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| StarGate Acceptor Vector pDSG-IBA33 | 5µg | 5-5209-001 | USD 260.00 | ||||
 StarGate Acceptor Vector designed for high-level stable transient expression in most mammalian hosts containing the following elements: ... |
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| StarGate Acceptor Vector pDSG-IBA43 | 5µg | 5-5211-001 | USD 260.00 | ||||
 StarGate Acceptor Vector designed for high-level stable transient expression in most mammalian hosts containing the following elements: ... |
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| StarGate Acceptor Vector pDSG-IBA45 | 5µg | 5-5214-001 | USD 260.00 | ||||
 StarGate Acceptor Vector designed for high-level stable transient expression in most mammalian hosts containing the following elements: ... |
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| StarGate Acceptor Vector pDSG-IBA102 | 5µg | 5-5219-001 | USD 260.00 | ||||
 StarGate Acceptor Vector designed for high-level stable transient expression in most mammalian hosts containing the following elements: ... |
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| StarGate Acceptor Vector pDSG-IBA103 | 5µg | 5-5220-001 | USD 260.00 | ||||
 StarGate Acceptor Vector designed for high-level stable transient expression in most mammalian hosts containing the following elements: ... |
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