Satapathy et al. demonstrate the utility of IBA’s MEXi system for mammalian expression of fully functional heavily post-translationally modified and structurally complex proteins, enabling the comprehensive analysis of structure-function relationships of difficult-to-express, low abundant or even secreted targets. After protein production using MEXi-293E cells in combination with our specially prepared transfection and culture media, they were able to obtain highly pure and functional mammalian chaperone clusterin upon elution from the Strep-Tactin®XT Superflow® high capacity cartridge. As compared to prior expression and purification strategies, the researchers report a more than six times substantial increase in yield directly from human plasma cultures, deploying a straightforward single-step chromatography protocol. Further, they made use of the integrated Strep-tag® toolbox by applying our StrepMAB-Classic antibody and Strep-Tactin® HRP conjugate for detection by cell staining as well as western blotting.
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#Mexi cells #CM and TM media #Streptactin-XT Superflow high capacity cartridge #StrepMAB-Classic# #2-6001-001 #2-6010-010 #2-6011-010 #2-4025-001 #2-1507-001