Protein purification with Strep-Tactin®
Strep-Tactin® allows affinity purification of Strep-tag®II and Twin-Strep-tag® fusion proteins under physiological conditions. In contrast to other tags, these mild purification parameters preserve bioactivity of the protein and the high specificity between Strep-tag ®II and Strep-Tactin ® can yield over 99% purity after a single chromatographical step.
Strep-Tactin® is especially suitable for standard proteins with good expression rates and binding properties. Furthermore, Strep-Tactin® can be alternatively used for the purification of proteins where the binding to the high affinity variant Strep-Tactin®XT is too strong.
Strep-Tactin® purification cycle
Strep-Tactin® resin is used for the purification of Strep-tag®II or Twin-Strep-tag® proteins. Protein purification with Strep-Tactin® is straightforward and quick, as the purification cycle for each target protein remains consistent. Additionally, if a target protein requires a specific buffer composition, such as PBS, HEPES, or buffer free from chelating agents like EDTA, the buffers can be easily adapted without altering the purification cycle. Strep-Tactin® resin is compatible with various buffer compositions, reagents, and additives. A comprehensive list of compatible reagents can be found here.
Strep-Tactin® |
|
Binding affinity |
Strep-tag® II: μM range Twin-Strep-tag®: nM range |
Elution |
Elution with Buffer E (2.5 mM Desthiobiotin) |
Regeneration |
Regeneration with 100 mM NaOH |
Find out more about Strep-tag® protein purification
Here, we have grouped links to make it easier to find more information about Strep-tag® technology for protein purification. In our web shop, you can find the protein purification products that fit your research needs. Read the comprehensive comparison of the most used tag systems, Strep-tag® vs. His-tag. Browser through application examples and frequently asked questions about Strep-tag® purification system or download application notes in our download area.
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