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Protein Detection with Strep-Tactin® Conjugates

Strep-Tactin® conjugates are available as:

  • Strep-Tactin® HRP conjugate (HRP, horseradish peroxidase)
  • Strep-Tactin® AP conjugate (AP, alkaline phosphatase)
  • Strep-Tactin® fluorescent conjugates for different wavelength

The HRP-conjugate is used for direct detection of Strep-tag® fusion proteins in Western blots via chromogenic and/or chemiluminescence reaction. The AP-conjugate is used for direct detection of Strep-tag® fusion proteins in Western blots via the chromogenic reaction. No secondary antibody needed!

Strep-Tactin® HRP conjugate

For chemiluminescence or chromogenic detection of Strep-tag® proteins on Western blots, we offer:

  • Strep-Tactin® horseradish peroxidase (HRP) conjugate (2-1502-001) for direct detection without the need for a secondary antibody. For details please refer to the Western blot protocol.
Strep-Tactin® AP conjugate

For chromogenic detection of Strep-tag® proteins on Western blots or ELISA we offer:

  • Strep-Tactin® alkaline phosphatase (AP) conjugate for direct detection without the need for a secondary antibody.
Fluorescent Strep-Tactin® conjugates

For fluorescent detection of Strep-tag® proteins in Immunocytochemistry and immunohistochemistry as well as FACS analysis.

Available as single conjugates of 50µg for different wavelengths:

  • Strep-Tactin® Chromeo 488
  • Strep-Tactin® Chromeo 546
  • Strep-Tactin® Oyster 645

 

Strep-Tactin® Detection Products

Product Contents Cat.no Price   Order
Biotin Blocking Buffer 2 ml 2-0501-002 USD 78.00  
2-0501-002

Biotin Blocking Buffer is recommended for blocking biotinylated proteins on Western blots prior to detection of Strep-tag® proteins with the Strep-Tactin® AP conjugate or Strep-Tactin® HRP conjugate.

Further details, manuals, data sheets, related products and more
Strep-Tactin®-HRP conjugate 0.5 ml 2-1502-001 USD 145.00  
2-1502-001

Strep-Tactin® HRP Conjugate (Strep-Tactin® labeled with horseradish peroxidase) is used for fast and direct detection of Strep-tag®II fusion proteins (N- or C-terminal or internal Strep-tag®II) transferred to a blotting membrane or coated to a microplate. Strep-Tactin® HRP Conjugate is suitable for chromogenic and chemiluminescence reaction.

Further details, manuals, data sheets, related products and more
Strep-Tactin® AP conjugate 0.5 ml 2-1503-001 USD 145.00  
2-1503-001

Strep-Tactin® AP Conjugate (Strep-Tactin® labeled with alkaline phosphatase) is used for fast and direct detection of Strep-tag®II fusion proteins (N- or C-terminal or internal Strep-tag®II) transferred to a blotting membrane or coated to a microplate. Strep-Tactin® AP Conjugate is suitable for chromogenic and chemiluminescence reaction.

Further details, manuals, data sheets, related products and more
Strep-Tactin Chromeo 488 conjugate 50µg 2-1542-050 USD 244.00
2-1542-050

For the detection of Strep-tag® II and Twin-Strep-tag in Immunofluorescence, FACS and more. Contains Strep-Tactin®, conjugated to Chromeo 488.1 x 50µg fluorescent conjugate. ...

Further details, manuals, data sheets, related products and more
Strep-Tactin Chromeo 546 conjugate 50µg 2-1548-050 USD 244.00
2-1548-050

For the detection of Strep-tag® II and Twin-Strep-tag in Immunofluorescence, FACS and more. Contains Strep-Tactin®, conjugated to Chromeo546.1 x 50µg fluorescent conjugate.

Further details, manuals, data sheets, related products and more
Strep-Tactin Oyster 645 conjugate 50µg 2-1553-050 USD 244.00
2-1553-050

For the detection of Strep-tag® II and Twin-Strep-tag in Immunofluorescence, FACS and more. Contains Strep-Tactin®, conjugated to Oyster 645.1 x 50µg fluorescent conjugate.

Further details, manuals, data sheets, related products and more

 

References for Detection with Strep-Tactin® Conjugates

  1. G. Hassaine, C. Deluz, X.-D. Li, A. Graff, H. Vogel, H. Nury (2013)
    Large scale expression and purification of the mouse 5-HT3 receptor
    Biochimica et Biophysica Acta (BBA) - Biomembranes, http://dx.doi.org/10.1016/j.bbamem.2013.05.028.
  2. Ernst, W.J., Spenger, A., Toellner, L., Katinger, H. and Grabherr, R.M. (2000) 
    Expanding baculovirus surface display modification of the native coat protein Gp64 of autographa californica NPV. 
    Eur. J. Biochem. 267, 4033-4039.
  3. Panke, O., Gumbiowski, K., Junge, W. and Engelbrecht, S. (2000) 
    F-ATPase: specific observation of the rotating c subunit oligomer of EFoEF1. 
    FEBS Letters 472, 34-38.
  4. Skerra, A. and Schmidt, T.G.M. (1993) 
    Use of the strep-tag and streptavidin for detection and purification of recombinant proteins. 
    Methods in Enzymology 326, 271-304.
  5. Sebastian, P., Wallwitz, J. and Schmidt, S. (2003) 
    Semi automated production of a set of different recombinant GST-streptag fusion proteins.
    J. Chromatogr. B 786, 343-355