Protein Detection with Strep-Tactin® Conjugates
Strep-Tactin® conjugates are available as:
- Strep-Tactin® HRP conjugate (HRP, horseradish peroxidase)
- Strep-Tactin® AP conjugate (AP, alkaline phosphatase)
- Strep-Tactin® fluorescent conjugates for different wavelength
The HRP-conjugate is used for direct detection of Strep-tag® fusion proteins in Western blots via chromogenic and/or chemiluminescence reaction. The AP-conjugate is used for direct detection of Strep-tag® fusion proteins in Western blots via the chromogenic reaction. No secondary antibody needed!
For chemiluminescence or chromogenic detection of Strep-tag® proteins on Western blots, we offer:
- Strep-Tactin® horseradish peroxidase (HRP) conjugate (2-1502-001) for direct detection without the need for a secondary antibody. For details please refer to the Western blot protocol.
For chromogenic detection of Strep-tag® proteins on Western blots or ELISA we offer:
- Strep-Tactin® alkaline phosphatase (AP) conjugate for direct detection without the need for a secondary antibody.
For fluorescent detection of Strep-tag® proteins in Immunocytochemistry and immunohistochemistry.
Available as single conjugates of 50µg for different wavelengths:
- Strep-Tactin® Chromeo 488
- Strep-Tactin® Chromeo 546
- Strep-Tactin® Oyster 645
Strep-Tactin® Detection Products
| Product | Contents | Cat.no | Price | Order | |||
|---|---|---|---|---|---|---|---|
| Biotin Blocking Buffer | 2 ml | 2-0501-002 | USD 78.00 | ||||
 Biotin Blocking Buffer is recommended for blocking biotinylated proteins on Western blots prior to detection of Strep-tag® proteins with the Strep-Tactin® AP conjugate or Strep-Tactin® HRP conjugate. |
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| Strep-Tactin®-HRP conjugate | 0.5 ml | 2-1502-001 | USD 145.00 | ||||
 Strep-Tactin® HRP Conjugate (Strep-Tactin® labeled with horseradish peroxidase) is used for fast and direct detection of Strep-tag®II fusion proteins (N- or C-terminal or internal Strep-tag®II) transferred to a blotting membrane or coated to a microplate. Strep-Tactin® HRP Conjugate is suitable for chromogenic and chemiluminescence reaction. |
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| Strep-Tactin® AP conjugate | 0.5 ml | 2-1503-001 | USD 145.00 | ||||
 Strep-Tactin® AP Conjugate (Strep-Tactin® labeled with alkaline phosphatase) is used for fast and direct detection of Strep-tag®II fusion proteins (N- or C-terminal or internal Strep-tag®II) transferred to a blotting membrane or coated to a microplate. Strep-Tactin® AP Conjugate is suitable for chromogenic and chemiluminescence reaction. |
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| Strep-Tactin Chromeo 488 conjugate | 50µg | 2-1542-050 | USD 244.00 | ||||
 For the detection of Strep-tag® II and Twin-Strep-tag in Immunofluorescence, FACS and more. Contains Strep-Tactin®, conjugated to Chromeo 488.1 x 50µg fluorescent conjugate. ... |
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| Strep-Tactin Chromeo 546 conjugate | 50µg | 2-1548-050 | USD 244.00 | ||||
 For the detection of Strep-tag® II and Twin-Strep-tag in Immunofluorescence, FACS and more. Contains Strep-Tactin®, conjugated to Chromeo546.1 x 50µg fluorescent conjugate. |
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| Strep-Tactin Oyster 645 conjugate | 50µg | 2-1553-050 | USD 244.00 | ||||
 For the detection of Strep-tag® II and Twin-Strep-tag in Immunofluorescence, FACS and more. Contains Strep-Tactin®, conjugated to Oyster 645.1 x 50µg fluorescent conjugate. |
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References for Detection with Strep-Tactin® Conjugates
- G. Hassaine, C. Deluz, X.-D. Li, A. Graff, H. Vogel, H. Nury (2013)
Large scale expression and purification of the mouse 5-HT3 receptor
Biochimica et Biophysica Acta (BBA) - Biomembranes, http://dx.doi.org/10.1016/j.bbamem.2013.05.028. - Ernst, W.J., Spenger, A., Toellner, L., Katinger, H. and Grabherr, R.M. (2000)
Expanding baculovirus surface display modification of the native coat protein Gp64 of autographa californica NPV.
Eur. J. Biochem. 267, 4033-4039. - Panke, O., Gumbiowski, K., Junge, W. and Engelbrecht, S. (2000)
F-ATPase: specific observation of the rotating c subunit oligomer of EFoEF1.
FEBS Letters 472, 34-38. - Skerra, A. and Schmidt, T.G.M. (1993)
Use of the strep-tag and streptavidin for detection and purification of recombinant proteins.
Methods in Enzymology 326, 271-304. - Sebastian, P., Wallwitz, J. and Schmidt, S. (2003)
Semi automated production of a set of different recombinant GST-streptag fusion proteins.
J. Chromatogr. B 786, 343-355
