Strep-Tactin® Sepharose® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Sepharose® columnsare intended for the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins in gravity flow. The low percentage of agarose allows the purification of large proteins. For proteins with multiple tags, such as multimers, that cannot be eluted from Strep-Tactin®XT resins due to the increased affinity, Strep-Tactin® Sepharose® columns are a lower affinity alternative. Biotin will bind irreversibly to Strep-Tactin®, and we recommend masking free biotin with BioLock before sample application.
To reduce the waiting period during sample and buffer application, the use of the application aid WET FRED is recommended. For columns with a custom resin bed volume, use Strep-Tactin® Sepharose® 50% suspension.
If you have different requirements for your purification, please check the specifications of our other resins.
|Bead Size:||45 - 165 µm|
|Biotin Binding Capacity:||> 300 nmol/ml resin|
|Exclusion Limit:||3 x 10^7|
|Form:||Pre-packed gravity flow column|
|Possible Application:||Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins|
|Specificity:||Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin|
|Strep-tag®II Binding Capacity:||3 mg/ml|
|Stability:||6 months after shipping|