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2-1209-051

Strep-Tactin® Superflow® high capacity column

Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Please note that this product will be discontinued and replaced by Strep-Tactin® 4Flow® high capacity column. Strep-Tactin® Superflow® high capacity column will still be available until 31st October 2024 or as long as stock lasts.
Check out the features of the new resin here.

Strep-Tactin® Superflow® high capacity columns are ready-to-use for purifying strep-tagged proteins below 90 kDa via affinity chromatography. These gravity flow columns contain a highly cross-linked agarose coated with streptavidin variant Strep-Tactin®, which captures proteins fused to a Strep-tag®II or a Twin-Strep-tag®.  A straightforward purification principle results into a highly pure (>95%) protein preparation. After regeneration, the columns can be used multiple times.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow® high capacity. If biotinylated proteins are expected, use BioLock. Furthermore, if large (sample and/or buffer) volumes have to be applied, we recommend our WET FRED system.

Specifications

Bead Size: 60 - 160 µm spherical
Biotin Binding Capacity: > 900 nmol/ml resin
Dynamic Protein Binding Capacity: 7 mg/ml resin
Exclusion Limit: 6 x 10^6
Form: Pre-packed gravity flow column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support: 6% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

BioLock
Biotin blocking solution for lysates and culture supernatants
Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
Buffer R (10x)
Strep-Tactin® regeneration buffer with HABA

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