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2-1240-001

Strep-Tactin® Superflow® high capacity FPLC column

FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Quantity Unit price
1-4
€416.00*
5-9
€338.00*
From 10
€332.00*
Content
Strep-Tactin® Superflow® high capacity FPLC columns are ready-to-use columns for affinity chromatography. They are suitable for automated protein purification with HPLC/FPLC devices such as Äkta® systems (Cytiva). The FPLC columns contain a cross-linked agarose coupled to the ligand Strep-Tactin®, a streptavidin variant that binds to Strep-tag®II or Twin-Strep-tag® fusion proteins. This protein purification system is characterized by a high target purity of >95% and a stable performance even after several regeneration cycles.
For proteins larger than 90 kDa, we recommend using our Strep-Tactin®XT 4Flow® and Strep-Tactin®4Flow® high capacity FPLC columns. Have a look at our resin specification overview with further recommendations.
Please note that the name of this product has been changed from Strep-Tactin® Superflow® high capacity cartridge to Strep-Tactin® Superflow® high capacity FPLC column.

Specifications

Bead Size: 60 - 160 µm spherical
Biotin Binding Capacity: > 900 nmol/ml resin
Dynamic Protein Binding Capacity: 7 mg/ml resin
Exclusion Limit: 6 x 10^6
Form: Pre-packed column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support: 6% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

BioLock
Biotin blocking solution for lysates and culture supernatants
Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
Buffer R (10x)
Strep-Tactin® regeneration buffer with HABA

Related Products

Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
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Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins