Strep-Tactin® Superflow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC columns are ready-to-use columns for affinity chromatography. They are suitable for automated protein purification with HPLC/FPLC devices such as Äkta® systems (Cytiva). The FPLC columns contain a cross-linked agarose coupled to the ligand Strep-Tactin®, a streptavidin variant that binds to Strep-tag®II or Twin-Strep-tag® fusion proteins. This protein purification system is characterized by a high target purity of >95% and a stable performance even after several regeneration cycles.
For proteins larger than 90 kDa, we recommend using our Strep-Tactin®XT 4Flow® and Strep-Tactin®4Flow® high capacity FPLC columns. Have a look at our resin specification overview with further recommendations.
Please note that the name of this product has been changed from Strep-Tactin® Superflow® high capacity cartridge to Strep-Tactin® Superflow® high capacity FPLC column.
|Bead Size:||60 - 160 µm spherical|
|Biotin Binding Capacity:||> 900 nmol/ml resin|
|Dynamic Protein Binding Capacity:||7 mg/ml resin|
|Exclusion Limit:||6 x 10^6|
|Possible Application:||Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins|
|Specificity:||Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin|
|Support:||6% agarose, crosslinked|
|Stability:||6 months after shipping|