5-1600-020

Competent E. coli TOP10

Chemically competent cells for plasmid propagation

€117.00*

Content

Competent E. coli TOP10 cells are ready for heat shock transformation with vector DNA and its subsequent propagation for cloning and transfection purposes. The chemically competent cells are provided as 20 separate one-shot reactions. Transformed cells can be selected by blue/white screening. 
The genotype of Competent E. coli TOP10 cells is F- mcrA (mrr-hsdRMS-mcrBC) 80lacZ M15 lacX74 recA1 ara 139 (ara-leu)7697 galU galK rpsL (StrR) endA1 nupG.

Specifications

Biosafety Level: S1 (Germany), BSL-2 (USA)
Form: Cryopreserved
Possible Application: Expression host for recombinat Strep-tag®II and Twin-Strep-tag® proteins

Shipping information

Storage: -80 °C
Stability: 12 months after shipping
Shipping: Dry ice

Accessories

pASK-IBA3C vector
Cytoplasmic E. coli expression vector encoding a C-terminal Strep-tag®II and chloramphenicol resistance
pASG-IBA3 vector
Cytoplasmic E. coli expression vector encoding a C-terminal Strep-tag®II
pDSG-IBA103 vector
Mammalian expression vector encoding a C-terminal Twin-Strep-tag®
pCSG-IBA3 vector
Mammalian expression vector encoding a C-terminal Strep-tag®II
pESG-IBA3 vector
Mammalian expression vector encoding a C-terminal Strep-tag®II
pLSG-IBA3 vector
Insect expression vector encoding a C-terminal Strep-tag®II
pYSG-IBA3 vector
Yeast expression vector encoding a C-terminal Strep-tag®II