Custom protein expression and purification

Our standard expression systems are E. coli and mammalian cells. Expression in yeast and insect cells (baculovirus) are available.

For request please download our questionnaire.

The expression procedure is divided into a test and a production phase.

Test Phase - expression is performed under standard conditions to evaluate the productivity of the expression system and to determine the culture volume necessary for the production of the amount of protein required by the customer.

Production Phase – expression and purification is performed in agreed culture volumes according to the yield of the recombinant protein as evaluated in the test phase.

E. coli expression

Test Phase (please refer to Table below)

• E. coli test culture (200 ml) and induction of protein expression

• Strep-Tactin® and/or Ni-NTA affinity purification, SDS-PAGE
• Estimation of the culture volume required to produce the requested amount of protein
• Time required: 1-2 weeks

Production Phase (please refer to Table below)

• E. coli culture in the appropriate volume, preparation of protein extract and Strep-Tactin® and/or Ni-NTA affinity purification

• Optimization (on request): A set of different E. coli test cultures (200 ml) under various expression conditions and/or different affinity purification attempts can be performed. This set of experiments will be planned in close consultation with the client and depends on the initial results of the E. coli test scale expression.

Mammalian expression

Test Phase (please refer to Table below)

• Transfection of mammalian cells
• Transient protein production for fast generation of small protein amounts
• Strep-Tactin® and/or Ni-NTA affinity purification from cell culture supernatant, SDS-PAGE
• Estimation of the required culture volume to obtain the requested amount of protein
• Time required: approximately 2 weeks

Production Phase (please refer to Table below)

• Transient transfection of mammalian cells is a powerful technology for the fast production of small amounts of recombinant proteins. Depending on adherent or suspension cells the customer can choose between 1L to 10L cultures.
• Strep-Tactin® and/or Ni-NTA affinity purification

General expression procedure

* Please note that standard affinity purification is performed with Strep-tag® and/or 6xHistidine-tag. Please get in touch with us to discuss your specific set-up.