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5-4505-001

pESG-IBA105 vector

Please note that this product will be discontinued at the end of 2026.

€279.00*

Content: 1 Piece

Prices excl. VAT plus shipping costs

Content
Stk
Please note that this product will be discontinued at the end of 2026.

The pESG-IBA105 vector is designed for high-level, stable, and non-replicative transient expression of target proteins with an N-terminal Twin-Strep-tag® in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

Affinity Tag N'-terminal: Twin-Strep-tag®
Cloning Method: Direct cloning using restriction enzyme Esp3I
Concentration: 250 ng/µl
Expression Host: Mammalian cells
Form: Suspension in TE buffer
Possible Application: Vector for recombinant expression in mammalian cells
Promoter: CMV Promoter
Resistance: Ampicillin, Neomycin
Sequence: See Documents
Size: 6163 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

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