Entry vector for generation of StarGate donor vectors
StarGate® Entry Vector is designed to insert the PCR product of a certain gene of interest (GOI) to generate a Donor Vector. StarGate cloning via the pENTRY-IBA vector provides a tool for the systematic and rapid screen of the best working expression conditions for a certain GOI, where nothing is known from literature. After initial pENTRY cloning, the GOI in the resulting Donor Vector can be easily transferred by a simple one-tube reaction into a multitude of expression vectors (Acceptor Vectors) providing different features.
|Cloning Method:||Direct cloning using restriction enzyme Esp3I|
|Expression Host:||E. coli|
|Form:||Suspension in TE buffer|
|Stability:||12 months after shipping|