5-4091-001

pENTRY-IBA51 vector

Entry vector for generation of StarGate donor vectors

€219.00*

Content
StarGate® Entry Vector is designed to insert the PCR product of a certain gene of interest (GOI) to generate a Donor Vector. StarGate cloning via the pENTRY-IBA vector provides a tool for the systematic and rapid screen of the best working expression conditions for a certain GOI, where nothing is known from literature. After initial pENTRY cloning, the GOI in the resulting Donor Vector can be easily transferred by a simple one-tube reaction into a multitude of expression vectors (Acceptor Vectors) providing different features.

Specifications

Cloning Method: Direct cloning using restriction enzyme Esp3I
Concentration: 250 ng/µl
Expression Host: E. coli
Form: Suspension in TE buffer
Promoter: tet Promoter
Resistance: Kanamycin
Sequence: See Documents
Size: 2556 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Competent E. coli TOP10
Chemically competent cells for plasmid propagation