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2-1257-001

Strep-Tactin® 4Flow® high capacity FPLC column

Replaces Strep-Tactin® Superflow® high capacity FPLC column
Content
Strep-Tactin® 4Flow® high capacity FPLC columns replace Strep-Tactin® Superflow® high capacity FPLC columns, providing identical performance and unifying the resins. Further information about the differences between Superflow® and 4Flow®, as well as the advantages of Strep-Tactin® 4Flow® high capacity, can be found here.

Strep-Tactin® 4Flow® high capacity FPLC columns are ready-to-use columns for affinity chromatography. They are suitable for automated protein purification with HPLC/FPLC devices such as Äkta® systems (Cytiva). The FPLC columns contain a cross-linked agarose coupled to the ligand Strep -Tactin®, a streptavidin variant that binds to Strep-tag®II or Twin-Strep-tag® fusion proteins. This protein purification system is characterized by a high target purity of >95% and a stable performance even after several regeneration cycles. These columns are the perfect alternative for proteins that are difficult to elute from Strep-Tactin®XT 4Flow® and Strep-Tactin®XT 4Flow® high capacity FPLC columns. Have a look at our resin specification overview with further recommendations.

Specifications

Bead Size: 50 - 150 µm spherical
Form: Pre-packed column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Protein Binding Capacity: 20 mg/ml resin
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

Buffer W (10x)
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
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Desthiobiotin
Desthiobiotin
Desthiobiotin powder for preparation of Strep-Tactin® elution buffer
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Buffer E (10x)
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
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Strep-Tactin® 4Flow® high capacity resin
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Empty Spin Columns
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