2-1000-025
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
Buffer E (10x) is a 10x concentrated Strep-Tactin® elution buffer. The contained desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® fusion proteins from the modified biotin-binding pocket of Strep-Tactin® in a competitive manner, resulting in a mild elution.
Please note that Buffer E (10x) is not applicable for elution from Strep-Tactin®XT resins.
Concentration: | 10x concentrated |
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Form: | Solution |
Possible Application: | Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin® resins |
Storage: | 2-8 °C |
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Stability: | 12 months after shipping |
Shipping: | Room temperature |