Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
Buffer E (10x) is a 10x concentrated Strep-Tactin® elution buffer. The contained desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® fusion proteins from the modified biotin-binding pocket of Strep-Tactin® in a competitive manner, resulting in a mild elution. Please note that Buffer E (10x) is not applicable for elution from Strep-Tactin®XT resins.
|Possible Application:||Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin® resins|
|Stability:||12 months after shipping|