As a result of the differences in binding strength among the possible tag-ligand combinations, the Strep-tag® system has become established as a universal tool for isolation of proteins and cells.
- Fused to recombinant proteins, the Strep-tag® enables efficient one-step purification on immobilized Strep-Tactin®.
- When fused to antibody-derived Fab fragments or nanobodies, Twin-Strep-tag® binds to multimerized Strep-Tactin® allowing capturing and releasing of target cells based on their surface-marker or antigen-specificity.
- Strep-Tactin® (or Strep-Tactin®XT), on its turn, can be conjugated to microplates, fluorophores or chips, allowing a wide range of analytical applications after isolation of target material, such as detection, immobilization and interaction studies.
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