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Specifications of Strep-Tactin® Conjugates Detection Reagents

Specifications of Strep-Tactin® HRP conjugate

Specifications of Strep-Tactin® HRP conjugate

Detection
N- or C-terminal or internal Strep-tag®II
Sensitivity in dot blots
> 7 pg/dot
Sensitivity in Western blots
> 7 pg/band
Cross reactivity
Reacts with biotin carboxyl carrier protein (22.5 kDa) in E. coli extracts. Generally, biotinylated proteins can be sufficiently masked by a 10 minute preincubation with Biotin Blocking Buffer.
Form
Suspended in 0.01 M phospate buffered saline pH 7.4 containing BSA and 0.01 % Thimerosal as preservative. Can be diluted 1:10,000 for detection of Strep-tag®II fusion proteins bound to microplates or 1:4,000 for detection of Strep-tag®II fusion proteins after Western blotting.
Stability
1 year
Storage
4 °C
Shipment
RT
 
Specifications of Strep-Tactin® AP conjugate

Specifications of Strep-Tactin® AP conjugate

Alkaline Phosphatase
Calf intestine
Detection
N- or C-terminal or internal Strep-tag®II
Sensitivity in dot blots
> 1 ng/dot
Sensitivity in Western blots
> 2 ng/band
Cross reactivity
Reacts with Biotin Carboxyl Carrier Protein (BCCP, 22.5 kDa) in E. coli extracts. Generally, biotinylated proteins can be sufficiently masked by a 10 minute preincubation with 2 µg/ml Biotin Blocking Buffer.
Form
Suspended in 0.01 M phospate buffered saline pH 7.4 containing BSA and 0.01 % Thimerosal as preservative. Can be diluted 1:4,000 for detection of Strep-tag®II fusion proteins after Western blotting. (Please note that for ELISA we recommend to use the Strep-Tactin® HRP conjugate)
Stability
1 year
Storage
4 °C
Shipment
RT
 
Fluorophores Chromeo 488, Chromeo 546, Oyster 645

Specifications of the fluorescent dyes:

Staining results strongly depend on the availability of the antigen, which may be improved with various fixation methods. For more information please click here.