2-1502-001
Strep-Tactin® HRP conjugate
Strep-Tactin® conjugate for detection of Strep-tag®II and Twin-Strep-tag® fusion proteins
The streptavidin variant Strep-Tactin® as conjugate with horseradish peroxidase (HRP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag®II or Twin-Strep-tag® fusion proteins transferred to a blotting membrane. Strep-Tactin® HRP is suitable for chromogenic and chemiluminescent reactions.
Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins, e.g., the biotin carboxyl carrier protein (BCCP, 22 kDa ) of E. coli. We recommend blocking such biotinylated proteins on western blots prior to detection of Strep-tag®II or Twin-Strep-tag® fusion proteins with Strep-Tactin® HRP to prevent the detection of false positive bands. For this purpose, use 2.5 mg/ml avidin in PBS. Avidin is a tetrameric biotin binding protein which selectively binds biotinylated proteins.
| Concentration: | 0.95-1.05 mg/ml |
|---|---|
| Form: | Suspension in PBS |
| Possible Application: | Western blot, detection in microplates |
| Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
GFP-Twin-Strep-tag® control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
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