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2-1502-001

Strep-Tactin® HRP conjugate

Strep-Tactin® conjugate for detection of Strep-tag®II and Twin-Strep-tag® fusion proteins

€121.00*

Content
The streptavidin variant Strep-Tactin® as conjugate with horseradish peroxidase (HRP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag®II or Twin-Strep-tag® fusion proteins transferred to a blotting membrane. Strep-Tactin® HRP is suitable for chromogenic and chemiluminescent reactions.
Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins, e.g., the biotin carboxyl carrier protein (BCCP, 22 kDa ) of E. coli. Biotin Blocking Buffer is recommended for blocking of such biotinylated proteins on western blots prior to detection of Strep-tag®II or Twin-Strep-tag® fusion proteins with Strep-Tactin® HRP to prevent the detection of false positive bands. For this purpose, Biotin Blocking Buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins.

Specifications

Concentration: 0.95-1.05 mg/ml
Form: Suspension in PBS
Possible Application: Western blot, detection in microplates
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Strep-tag®II peptide
Synthetic peptide recognized by Strep-Tactin® and Strep-Tactin®XT
GFP-Strep-tag®II control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
GFP-Twin-Strep-tag® control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
Biotin Blocking Buffer
Efficient blocking buffer for biotinylated proteins in western blot analyses

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