2-1209-550
Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity columns are ready-to-use for purifying strep-tagged proteins below 90 kDa via affinity chromatography. These gravity flow columns contain a highly cross-linked agarose coated with streptavidin variant Strep-Tactin®, which captures proteins fused to a Strep-tag®II or a Twin-Strep-tag®. A straightforward purification principle results into a highly pure (>95%) protein preparation. After regeneration, the columns can be used multiple times.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow® high capacity. If biotinylated proteins are expected, use BioLock. Furthermore, if large (sample and/or buffer) volumes have to be applied, we recommend our WET FRED system.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow® high capacity. If biotinylated proteins are expected, use BioLock. Furthermore, if large (sample and/or buffer) volumes have to be applied, we recommend our WET FRED system.
For a resin overview with recommendations, browse our resin specifications.
| Bead Size: | 60 - 160 µm spherical |
|---|---|
| Biotin Binding Capacity: | > 900 nmol/ml resin |
| Dynamic Protein Binding Capacity: | 7 mg/ml resin |
| Exclusion Limit: | 6 x 10^6 |
| Form: | Pre-packed gravity flow column |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
| Support: | 6% agarose, crosslinked |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Accessories
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