2-1209-550

Strep-Tactin® Superflow® high capacity column

Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

€256.00*

Content: 1 Stk
Content
Strep-Tactin® Superflow® high capacity columns contain a 6% agarose coupled with the streptavidin variant Strep-Tactin® and are applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins via gravity flow. In comparison to Strep-Tactin® Superflow®, Strep-Tactin® Superflow® high capacity has a higher density of immobilized Strep-Tactin®, which leads to a 3- to 5-fold higher yield of purified protein.
To reduce the waiting period during sample and buffer application, the use of the application aid WET FRED is recommended.

Specifications

Bead Size: 60 - 160 µm spherical
Biotin Binding Capacity: > 900 nmol/ml resin
Dynamic Protein Binding Capacity: 7 mg/ml resin
Exclusion Limit: 6 x 10^6
Form: Pre-packed gravity flow column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support: 6% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

BioLock
Biotin blocking solution for lysates and culture supernatants
Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
Buffer R (10x)
Strep-Tactin® regeneration buffer with HABA

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