2-1209-550
Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity columns contain a 6% agarose coupled with the streptavidin variant Strep-Tactin® and are applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins via gravity flow. In comparison to Strep-Tactin® Superflow®, Strep-Tactin® Superflow® high capacity has a higher density of immobilized Strep-Tactin®, which leads to a 3- to 5-fold higher yield of purified protein.
To reduce the waiting period during sample and buffer application, the use of the application aid WET FRED is recommended.
To reduce the waiting period during sample and buffer application, the use of the application aid WET FRED is recommended.
| Bead Size: | 60 - 160 µm spherical |
|---|---|
| Biotin Binding Capacity: | > 900 nmol/ml resin |
| Dynamic Protein Binding Capacity: | 7 mg/ml resin |
| Exclusion Limit: | 6 x 10^6 |
| Form: | Pre-packed gravity flow column |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
| Support: | 6% agarose, crosslinked |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Accessories
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