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2-1208-002

Strep-Tactin® Superflow® high capacity resin

50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

€169.00*

Content: 1 Stk
Content
Strep-Tactin® Superflow® high capacity is a resin for purification of strep-tagged proteins <90 kDa via affinity chromatography. The product is optimized for column-based use and suitable for HPLC/FPLC. It consists out of highly cross-linked agarose beads that are coupled to Strep-Tactin®, which is a streptavidin variant. Strep-Tactin® binds to proteins that were fused to a Strep-tag®II or a Twin-Strep-tag®, enabling a straightforward purification of tagged proteins from mixed samples, reaching a purity of >95%. Due to the targeted elution, tedious titration steps are not necessary. In addition, regeneration allows for multiple use of the resin.
In comparison to Strep-Tactin® Superflow, the high capacity version has a higher density of Strep-Tactin®, making it suitable for highly expressed proteins. This resin is also an alternative for proteins that are difficult to elute from our Strep-Tactin®XT 4Flow® resins.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow® high capacity. If biotinylated proteins are expected, use BioLock.  Furthermore, this resin is a 50% suspension, so for packing e.g. a 1 ml gravity column, 2 ml of resin is required. 

For further information about our different resins including recommendations, check our resin specifications.

Specifications

Bead Size: 60 - 160 µm spherical
Biotin Binding Capacity: > 900 nmol/ml resin
Dynamic Protein Binding Capacity: 7 mg/ml resin
Exclusion Limit: 6 x 10^6
Form: 50% suspension
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support: 6% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

BioLock
Biotin blocking solution for lysates and culture supernatants
Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
Buffer R (10x)
Strep-Tactin® regeneration buffer with HABA

Related Products

Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins