2-1208-002
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity is a 6% agarose coupled with the streptavidin variant Strep-Tactin® and applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. In comparison to Strep-Tactin® Superflow®, Strep-Tactin® Superflow® high capacity has a higher density of immobilized Strep-Tactin®, which leads to a 3- to 5-fold higher yield of purified protein.
The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension is necessary.
The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension is necessary.
| Bead Size: | 60 - 160 µm spherical |
|---|---|
| Biotin Binding Capacity: | > 900 nmol/ml resin |
| Dynamic Protein Binding Capacity: | 7 mg/ml resin |
| Exclusion Limit: | 6 x 10^6 |
| Form: | 50% suspension |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
| Support: | 6% agarose, crosslinked |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
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Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
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Strep-Tactin® Sepharose® resin
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50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
