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2-1206-025

Strep-Tactin® Superflow® resin

50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Please note that this product will be discontinued and replaced by Strep-Tactin® 4Flow® high capacity resin. Strep-Tactin® Superflow® resin will still be available until 31st October 2024 or as long as stock lasts.
Check out the features of the new resin here.

Strep-Tactin® Superflow® is suitable for affinity chromatography-based purification of strep-tagged fusion proteins from cell culture media, cell lysates or other mixed protein preparations. It is optimized for column affinity chromatography and recommended for proteins <90 kDa.
The resin consists out of highly cross-linked agarose beads coupled to Strep-Tactin®, a streptavidin variant that binds to proteins fused to a Strep-tag®II or a Twin-Strep-tag®. This purification system delivers an outstanding target purity (>95%) without tedious optimization steps due to the specific binding of tagged proteins and a subsequent targeted elution. Resin regeneration is possible, enabling multiple purification cycles.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow®. If biotinylated proteins are expected, use BioLock.  Furthermore, this resin is a 50% suspension, so for packing e.g. a 1 ml gravity column, 2 ml of resin is required.

Specifications

Bead Size: 60 - 160 µm spherical
Biotin Binding Capacity: > 300 nmol/ml resin
Exclusion Limit: 6 x 10^6
Form: 50% suspension
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Strep-tag®II Binding Capacity: 3 mg/ml
Support: 6% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

BioLock
Biotin blocking solution for lysates and culture supernatants
Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
Buffer R (10x)
Strep-Tactin® regeneration buffer with HABA

Related Products

Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins