Strep-Tactin® Superflow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® is suitable for affinity chromatography-based purification of strep-tagged fusion proteins from cell culture media, cell lysates or other mixed protein preparations. It is optimized for column affinity chromatography and recommended for proteins <90 kDa.
The resin consists out of highly cross-linked agarose beads coupled to Strep-Tactin®, a streptavidin variant that binds to proteins fused to a Strep-tag®II or a Twin-Strep-tag®. This purification system delivers an outstanding target purity (>95%) without tedious optimization steps due to the specific binding of tagged proteins and a subsequent targeted elution. Resin regeneration is possible, enabling multiple purification cycles.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow®. If biotinylated proteins are expected, use BioLock. Furthermore, this resin is a 50% suspension, so for packing e.g. a 1 ml gravity column, 2 ml of resin is required.
If you are unsure if this is the best resin for your approach, have a look at our resin specifications.
|Bead Size:||60 - 160 µm spherical|
|Biotin Binding Capacity:||> 300 nmol/ml resin|
|Exclusion Limit:||6 x 10^6|
|Possible Application:||Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins|
|Specificity:||Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin|
|Strep-tag®II Binding Capacity:||3 mg/ml|
|Support:||6% agarose, crosslinked|
|Stability:||6 months after shipping|